Isoespintanol, a monoterpene isolated from oxandra cf xylopioides, ameliorates the myocardial ischemia-reperfusion injury by AKT/PKCε/eNOS-dependent pathways

To determine the actions of isoespintanol (Isoesp) on post-ischemic myocardial and mitochondrial alterations. Hearts removed from Wistar rats were perfused by 20 min. After this period, the coronary flow was interrupted by half an hour and re-established during 1 h. In the treated group, Isoesp was...

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Autores principales:	González Arbeláez, Luisa Fernanda, Ciocci Pardo, Alejandro, Fantinelli, Juliana Catalina, Rojano, Benjamín Alberto, Schinella, Guillermo Raúl, Mosca, Susana María
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2019
Materias:	Ciencias Médicas Isoespintanol Ischemia-reperfusion Infarct size Akt PKCε eNOS
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/137707
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-137707
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Médicas Isoespintanol Ischemia-reperfusion Infarct size Akt PKCε eNOS
spellingShingle	Ciencias Médicas Isoespintanol Ischemia-reperfusion Infarct size Akt PKCε eNOS González Arbeláez, Luisa Fernanda Ciocci Pardo, Alejandro Fantinelli, Juliana Catalina Rojano, Benjamín Alberto Schinella, Guillermo Raúl Mosca, Susana María Isoespintanol, a monoterpene isolated from oxandra cf xylopioides, ameliorates the myocardial ischemia-reperfusion injury by AKT/PKCε/eNOS-dependent pathways
topic_facet	Ciencias Médicas Isoespintanol Ischemia-reperfusion Infarct size Akt PKCε eNOS
description	To determine the actions of isoespintanol (Isoesp) on post-ischemic myocardial and mitochondrial alterations. Hearts removed from Wistar rats were perfused by 20 min. After this period, the coronary flow was interrupted by half an hour and re-established during 1 h. In the treated group, Isoesp was administered at the beginning of reperfusion. To assess the participation of e isoform of protein kinase C (PKCe), protein kinase B (PKB/Akt), and nitric oxide synthase (NOS), hearts were treated with Isoesp plus the respective inhibitors (chelerythrine, wortmannin, and N-nitro-l-arginine methyl ester). Cell death was determined by triphenyl tetrazolium chloride staining technique. Post-ischemic recovery of contractility, oxidative stress, and content of phosphorylated forms of PKCe, Akt, and eNOS were also examined. Mitochondrial state was assessed through the measurement of calcium-mediated response, calcium retention capacity, and mitochondrial potential. Isoesp limited cell death, decreased post-ischemic dysfunction and oxidative stress, improved mitochondrial state, and increased the expression of PKCe, Akt, and eNOS phosphorylated. All these beneficial effects achieved by Isoesp were annulled by the inhibitors. These findings suggest that activation of Akt/eNOS and PKCe signaling pathways are involved in the development of Isoesp-induced cardiac and mitochondria tolerance to ischemia-reperfusion.
format	Articulo Articulo
author	González Arbeláez, Luisa Fernanda Ciocci Pardo, Alejandro Fantinelli, Juliana Catalina Rojano, Benjamín Alberto Schinella, Guillermo Raúl Mosca, Susana María
author_facet	González Arbeláez, Luisa Fernanda Ciocci Pardo, Alejandro Fantinelli, Juliana Catalina Rojano, Benjamín Alberto Schinella, Guillermo Raúl Mosca, Susana María
author_sort	González Arbeláez, Luisa Fernanda
title	Isoespintanol, a monoterpene isolated from oxandra cf xylopioides, ameliorates the myocardial ischemia-reperfusion injury by AKT/PKCε/eNOS-dependent pathways
title_short	Isoespintanol, a monoterpene isolated from oxandra cf xylopioides, ameliorates the myocardial ischemia-reperfusion injury by AKT/PKCε/eNOS-dependent pathways
title_full	Isoespintanol, a monoterpene isolated from oxandra cf xylopioides, ameliorates the myocardial ischemia-reperfusion injury by AKT/PKCε/eNOS-dependent pathways
title_fullStr	Isoespintanol, a monoterpene isolated from oxandra cf xylopioides, ameliorates the myocardial ischemia-reperfusion injury by AKT/PKCε/eNOS-dependent pathways
title_full_unstemmed	Isoespintanol, a monoterpene isolated from oxandra cf xylopioides, ameliorates the myocardial ischemia-reperfusion injury by AKT/PKCε/eNOS-dependent pathways
title_sort	isoespintanol, a monoterpene isolated from oxandra cf xylopioides, ameliorates the myocardial ischemia-reperfusion injury by akt/pkcε/enos-dependent pathways
publishDate	2019
url	http://sedici.unlp.edu.ar/handle/10915/137707
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Isoespintanol, a monoterpene isolated from oxandra cf xylopioides, ameliorates the myocardial ischemia-reperfusion injury by AKT/PKCε/eNOS-dependent pathways

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