Embryo rescue and induction of somatic embryogenesis as a method to overcome seed inviability in Zea mays ssp. mays × Zea mays ssp. parviglumis crosses

Zea mays ssp. mays (2n=40) and Z. mays ssp. parviglumis (2n=20) were crossed to obtain hybrid plants by embryo rescue. Hybrid embryos were isolated and cultured on Garcia et al. (1992) basic medium supplemented with 2,4-dichlorophenoxyacetic acid and/or kinetin in different concentrations. Caryopses...

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Detalles Bibliográficos
Autores principales: García, Maria Dina, Molina, María del Carmen
Formato: Articulo
Lenguaje:Inglés
Publicado: 2001
Materias:
Ciencias Agrarias
2,4-dichlorophenoxyacetic acid
Incompatible crosses
Maize hybrids
Organogenesis
Plant regeneration
Tissue culture
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/137704
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-137704
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Agrarias
2,4-dichlorophenoxyacetic acid
Incompatible crosses
Maize hybrids
Organogenesis
Plant regeneration
Tissue culture
spellingShingle Ciencias Agrarias
2,4-dichlorophenoxyacetic acid
Incompatible crosses
Maize hybrids
Organogenesis
Plant regeneration
Tissue culture
García, Maria Dina
Molina, María del Carmen
Embryo rescue and induction of somatic embryogenesis as a method to overcome seed inviability in Zea mays ssp. mays × Zea mays ssp. parviglumis crosses
topic_facet Ciencias Agrarias
2,4-dichlorophenoxyacetic acid
Incompatible crosses
Maize hybrids
Organogenesis
Plant regeneration
Tissue culture
description Zea mays ssp. mays (2n=40) and Z. mays ssp. parviglumis (2n=20) were crossed to obtain hybrid plants by embryo rescue. Hybrid embryos were isolated and cultured on Garcia et al. (1992) basic medium supplemented with 2,4-dichlorophenoxyacetic acid and/or kinetin in different concentrations. Caryopses harvested 23 d after pollination (DAP) were turgid, with 0.3 to 0.5 mm long embryos, while those harvested 30 DAP were shrunken, with 1 to 1.5 mm long embryos. Twenty days after plating, 100 % of the younger embryos gave rise to white, compact embryogenic calli. Subsequently, coleoptiles, leaf-like structures, shoots and roots originated from them and 35 hybrid plants were regenerated from 60 embryos. Embryogenic or organogenic calli frequencies did not differ among hormonal treatments, but they decreased, on average, from 90.5 to 44.3 %, comparing 50 and 120-d-old cultures. The older embryos regenerated plants only by germination, although they gave rise to organogenic callus with low frequencies. Regenerated plants showed a somatic chromosome number of 2n=30, pollen fertility of 40 to 80 % and 15 % viable naked caryopses.
format Articulo
Articulo
author García, Maria Dina
Molina, María del Carmen
author_facet García, Maria Dina
Molina, María del Carmen
author_sort García, Maria Dina
title Embryo rescue and induction of somatic embryogenesis as a method to overcome seed inviability in Zea mays ssp. mays × Zea mays ssp. parviglumis crosses
title_short Embryo rescue and induction of somatic embryogenesis as a method to overcome seed inviability in Zea mays ssp. mays × Zea mays ssp. parviglumis crosses
title_full Embryo rescue and induction of somatic embryogenesis as a method to overcome seed inviability in Zea mays ssp. mays × Zea mays ssp. parviglumis crosses
title_fullStr Embryo rescue and induction of somatic embryogenesis as a method to overcome seed inviability in Zea mays ssp. mays × Zea mays ssp. parviglumis crosses
title_full_unstemmed Embryo rescue and induction of somatic embryogenesis as a method to overcome seed inviability in Zea mays ssp. mays × Zea mays ssp. parviglumis crosses
title_sort embryo rescue and induction of somatic embryogenesis as a method to overcome seed inviability in zea mays ssp. mays × zea mays ssp. parviglumis crosses
publishDate 2001
url http://sedici.unlp.edu.ar/handle/10915/137704
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AT molinamariadelcarmen embryorescueandinductionofsomaticembryogenesisasamethodtoovercomeseedinviabilityinzeamayssspmayszeamayssspparviglumiscrosses
bdutipo_str Repositorios
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