Lipid binding properties of a factor necessary for linoleic acid desaturation

Suspension and centrifugation of crude microsomes of rat liver in low ionic strength solution separated a soluble protein fraction that is necessary for the full activity of the linoleic acid desaturase. The fraction partially purified through Sephadex G-150 still retains lipids which are mainly con...

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Detalles Bibliográficos
Autores principales: Leikin, Alicia Isabel, Nervi, Aníbal M., Brenner, Rodolfo Roberto
Formato: Articulo
Lenguaje:Inglés
Publicado: 1979
Materias:
Bioquímica
Lipid
Linoleic acid
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/137522
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-137522
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Lipid
Linoleic acid
spellingShingle Bioquímica
Lipid
Linoleic acid
Leikin, Alicia Isabel
Nervi, Aníbal M.
Brenner, Rodolfo Roberto
Lipid binding properties of a factor necessary for linoleic acid desaturation
topic_facet Bioquímica
Lipid
Linoleic acid
description Suspension and centrifugation of crude microsomes of rat liver in low ionic strength solution separated a soluble protein fraction that is necessary for the full activity of the linoleic acid desaturase. The fraction partially purified through Sephadex G-150 still retains lipids which are mainly constituted by phosphatidylcholine. Linoleic acid predominates in the fatty acid composition. By NaCl gradient centrifugation and electrophoresis in gelatinized cellulose acetate, the factor behaves like a lipoprotein. The factor binds linoleic acid and linolyl-CoA that are desaturated to γ-linolenic acid when incubated with washed microsomes. Albumin does not replace the factor.
format Articulo
Articulo
author Leikin, Alicia Isabel
Nervi, Aníbal M.
Brenner, Rodolfo Roberto
author_facet Leikin, Alicia Isabel
Nervi, Aníbal M.
Brenner, Rodolfo Roberto
author_sort Leikin, Alicia Isabel
title Lipid binding properties of a factor necessary for linoleic acid desaturation
title_short Lipid binding properties of a factor necessary for linoleic acid desaturation
title_full Lipid binding properties of a factor necessary for linoleic acid desaturation
title_fullStr Lipid binding properties of a factor necessary for linoleic acid desaturation
title_full_unstemmed Lipid binding properties of a factor necessary for linoleic acid desaturation
title_sort lipid binding properties of a factor necessary for linoleic acid desaturation
publishDate 1979
url http://sedici.unlp.edu.ar/handle/10915/137522
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bdutipo_str Repositorios
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