Release of outer membrane vesicles from Bordetella pertussis

The aim of the study reported here was to investigate the production of Bordetella pertussis outer membrane vesicles (OMVs). Numerous vesicles released from cells grown in Stainer-Scholte liquid medium were observed. The formation of similar vesicle-like structures could also be artificially induced...

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Detalles Bibliográficos
Autores principales: Hozbor, Daniela Flavia, Rodríguez, María Eugenia, Fernández, Julieta, Lagares, Antonio, Guiso, Nicole, Yantorno, Osvaldo Miguel
Formato: Articulo
Lenguaje:Inglés
Publicado: 1999
Materias:
Biología
Polypeptide
Liquid Medium
Outer Membrane
Basic Material
Pertussis
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/137514
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-137514
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Polypeptide
Liquid Medium
Outer Membrane
Basic Material
Pertussis
spellingShingle Biología
Polypeptide
Liquid Medium
Outer Membrane
Basic Material
Pertussis
Hozbor, Daniela Flavia
Rodríguez, María Eugenia
Fernández, Julieta
Lagares, Antonio
Guiso, Nicole
Yantorno, Osvaldo Miguel
Release of outer membrane vesicles from Bordetella pertussis
topic_facet Biología
Polypeptide
Liquid Medium
Outer Membrane
Basic Material
Pertussis
description The aim of the study reported here was to investigate the production of Bordetella pertussis outer membrane vesicles (OMVs). Numerous vesicles released from cells grown in Stainer-Scholte liquid medium were observed. The formation of similar vesicle-like structures could also be artificially induced by sonication of concentrated bacterial suspensions. Immunoblot analysis showed that OMVs contain adenylate cyclase-hemolysin (AC-Hly), among other polypeptides, as well as the lipopolysaccharide (LPS). Experiments carried out employing purified AC-Hly and OMVs isolated from B. pertussis AC-Hly− showed that AC-Hly is an integral component of the vesicles. OMVs reported here contain several protective immunogens and might be considered a possible basic material for the development of acellular pertussis vaccines.
format Articulo
Articulo
author Hozbor, Daniela Flavia
Rodríguez, María Eugenia
Fernández, Julieta
Lagares, Antonio
Guiso, Nicole
Yantorno, Osvaldo Miguel
author_facet Hozbor, Daniela Flavia
Rodríguez, María Eugenia
Fernández, Julieta
Lagares, Antonio
Guiso, Nicole
Yantorno, Osvaldo Miguel
author_sort Hozbor, Daniela Flavia
title Release of outer membrane vesicles from Bordetella pertussis
title_short Release of outer membrane vesicles from Bordetella pertussis
title_full Release of outer membrane vesicles from Bordetella pertussis
title_fullStr Release of outer membrane vesicles from Bordetella pertussis
title_full_unstemmed Release of outer membrane vesicles from Bordetella pertussis
title_sort release of outer membrane vesicles from bordetella pertussis
publishDate 1999
url http://sedici.unlp.edu.ar/handle/10915/137514
work_keys_str_mv AT hozbordanielaflavia releaseofoutermembranevesiclesfrombordetellapertussis
AT rodriguezmariaeugenia releaseofoutermembranevesiclesfrombordetellapertussis
AT fernandezjulieta releaseofoutermembranevesiclesfrombordetellapertussis
AT lagaresantonio releaseofoutermembranevesiclesfrombordetellapertussis
AT guisonicole releaseofoutermembranevesiclesfrombordetellapertussis
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bdutipo_str Repositorios
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