Sarcocystis spp. infection in South American deer huemul (Hippocamelus bisulcus) and pudu (Pudu puda) from Patagonian National Parks, Argentina

Sarcocystis spp. are intracellular protozoan parasites with heteroxenous life cycles. This study described Sarcocystis spp. infection in adult South American native deer huemul (Hippocamelus bisulcus) and pudu (Pudu puda). Heart, diaphragm, tongue, and skeletal muscle samples were collected from 5 h...

Descripción completa

Detalles Bibliográficos
Autores principales: Chang Reissig, Elizabeth, Helman, Elisa, Moré, Gastón Andrés
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2020
Materias:
Ciencias Veterinarias
Sarcocystis
Ultrastructure
PCR-sequencing
Native cervids/wild ungulates
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/137210
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-137210
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Sarcocystis
Ultrastructure
PCR-sequencing
Native cervids/wild ungulates
spellingShingle Ciencias Veterinarias
Sarcocystis
Ultrastructure
PCR-sequencing
Native cervids/wild ungulates
Chang Reissig, Elizabeth
Helman, Elisa
Moré, Gastón Andrés
Sarcocystis spp. infection in South American deer huemul (Hippocamelus bisulcus) and pudu (Pudu puda) from Patagonian National Parks, Argentina
topic_facet Ciencias Veterinarias
Sarcocystis
Ultrastructure
PCR-sequencing
Native cervids/wild ungulates
description Sarcocystis spp. are intracellular protozoan parasites with heteroxenous life cycles. This study described Sarcocystis spp. infection in adult South American native deer huemul (Hippocamelus bisulcus) and pudu (Pudu puda). Heart, diaphragm, tongue, and skeletal muscle samples were collected from 5 huemuls and 2 pudus, found dead in National Parks. Direct microscopic examination, transmission electron microscopy, PCR, and sequencing were performed. Sarcocystis spp. microscopic thin-walled cysts were identified in 3 huemuls and 1 pudu. Several cysts from 1 huemul and 1 pudu were observed by TEM; ultrastructure was similar to previously reported as cyst wall type 17 and types 2 and 8, respectively. Fragments of the 18S rRNA and cytochrome c oxidase subunit I (cox1) genes were amplified and sequenced from 3 individual cysts from 2 huemuls and 2 cysts from the pudu. The sequences from huemuls showed a high identity among them (> 99%) at both amplified targets. The highest identities were > 99.7% at 18S rRNA and 93% at cox1 with S. tarandivulpes sequences. The 18S rRNA gene sequences from pudus showed an identity > 99.7% with Sarcocystis sp., S. taeniata, and S. linearis sequences, while the cox1 sequences were different, one showing 99.42% identity with S. venatoria and the other 98.22% with S. linearis. A single species, similar to S. tarandivulpes, was identified in all huemul samples while 2 molecularly different Sarcocystis spp. were found in 1 pudu with high similarities to either S. venatoria or to S. linearis, S. taeniata-like, and S. morae. Based on the cox1 sequence identities, at least the Sarcocystis sp. in huemuls might represent a new species, primarily occurring in this host. Additional sarcocyst isolates from both hosts need to be examined molecularly in order to firmly establish whether these species are indeed native to huemuls and/or pudus or are derived from introduced deer species.
format Articulo
Comunicacion
author Chang Reissig, Elizabeth
Helman, Elisa
Moré, Gastón Andrés
author_facet Chang Reissig, Elizabeth
Helman, Elisa
Moré, Gastón Andrés
author_sort Chang Reissig, Elizabeth
title Sarcocystis spp. infection in South American deer huemul (Hippocamelus bisulcus) and pudu (Pudu puda) from Patagonian National Parks, Argentina
title_short Sarcocystis spp. infection in South American deer huemul (Hippocamelus bisulcus) and pudu (Pudu puda) from Patagonian National Parks, Argentina
title_full Sarcocystis spp. infection in South American deer huemul (Hippocamelus bisulcus) and pudu (Pudu puda) from Patagonian National Parks, Argentina
title_fullStr Sarcocystis spp. infection in South American deer huemul (Hippocamelus bisulcus) and pudu (Pudu puda) from Patagonian National Parks, Argentina
title_full_unstemmed Sarcocystis spp. infection in South American deer huemul (Hippocamelus bisulcus) and pudu (Pudu puda) from Patagonian National Parks, Argentina
title_sort sarcocystis spp. infection in south american deer huemul (hippocamelus bisulcus) and pudu (pudu puda) from patagonian national parks, argentina
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/137210
work_keys_str_mv AT changreissigelizabeth sarcocystissppinfectioninsouthamericandeerhuemulhippocamelusbisulcusandpudupudupudafrompatagoniannationalparksargentina
AT helmanelisa sarcocystissppinfectioninsouthamericandeerhuemulhippocamelusbisulcusandpudupudupudafrompatagoniannationalparksargentina
AT moregastonandres sarcocystissppinfectioninsouthamericandeerhuemulhippocamelusbisulcusandpudupudupudafrompatagoniannationalparksargentina
bdutipo_str Repositorios
_version_ 1764820456622260225

Ejemplares similares