<i>Neospora caninum</i> NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams

<i>Neospora caninum</i> infection is a major cause of abortion in cattle. The objectives of this study were to genetically characterize the <i>N. caninum</i> NC-6 Argentina isolate using a multilocus microsatellite analysis approach and to study its biological behavior by exp...

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Autores principales: Bacigalupe, Diana Raquel, Basso, Walter Ubaldo, Caspe, S. G., Moré, Gastón Andrés, Lischinsky, L., Gos, María Laura, Leunda, María Rosa, Campero, Lucía María, Moore, Dadín Prando, Schares, Gereon, Campero, Carlos Manuel, Venturini, María Cecilia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2013
Materias:
Veterinaria
Cellular Immune Response
Brucellosis
Bovine Viral Diarrhea Virus
Seropositive Animal
Transplacental Transmission
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/137194
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-137194
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Veterinaria
Cellular Immune Response
Brucellosis
Bovine Viral Diarrhea Virus
Seropositive Animal
Transplacental Transmission
spellingShingle Veterinaria
Cellular Immune Response
Brucellosis
Bovine Viral Diarrhea Virus
Seropositive Animal
Transplacental Transmission
Bacigalupe, Diana Raquel
Basso, Walter Ubaldo
Caspe, S. G.
Moré, Gastón Andrés
Lischinsky, L.
Gos, María Laura
Leunda, María Rosa
Campero, Lucía María
Moore, Dadín Prando
Schares, Gereon
Campero, Carlos Manuel
Venturini, María Cecilia
<i>Neospora caninum</i> NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams
topic_facet Veterinaria
Cellular Immune Response
Brucellosis
Bovine Viral Diarrhea Virus
Seropositive Animal
Transplacental Transmission
description <i>Neospora caninum</i> infection is a major cause of abortion in cattle. The objectives of this study were to genetically characterize the <i>N. caninum</i> NC-6 Argentina isolate using a multilocus microsatellite analysis approach and to study its biological behavior by experimental inoculations into seronegative and seropositive pregnant cattle, evaluating the humoral and cellular immune response elicited and the occurrence of transplacental transmission and fetopathy. Pregnant cows (65 days of gestation) seropositive and seronegative to <i>N. caninum</i> were intravenously inoculated with tachyzoites of the NC-6 Argentina <i>N. caninum</i> strain and slaughtered at 108 ± 2 days of gestation. Serum samples were analyzed for <i>N. caninum</i> antibodies by indirect fluorescent antibody test. The cellular immune response was analyzed by detection of gamma interferon (γIFN) production in blood cells. Tissue samples from dams, fetuses, and placental cotyledons were processed by histopathological and immunohistochemical techniques and examined for <i>N. caninum</i> DNA by PCR. Positive DNA samples were further analyzed by multilocus microsatellite typing for <i>N. caninum</i>. Inoculated animals had significantly higher <i>N. caninum</i> antibody titers and γIFN production than control animals. One seropositive inoculated cow aborted, one seronegative cow had a non-viable fetus, and the remaining fetuses from the experimentally inoculated dams had histopathologic lesions. The PCR was positive in 3/4 fetuses from seronegative inoculated cows and in 2/3 fetuses from seropositive inoculated cows. Multilocus microsatellite analysis revealed that the <i>N. caninum</i> DNA present in fetuses and placentas had an identical pattern to NC-6 Argentina strain. The NC-6 Argentina strain proved to be able to cross the placenta and to induce fetopathy in both the seropositive and seronegative dams.
format Articulo
Articulo
author Bacigalupe, Diana Raquel
Basso, Walter Ubaldo
Caspe, S. G.
Moré, Gastón Andrés
Lischinsky, L.
Gos, María Laura
Leunda, María Rosa
Campero, Lucía María
Moore, Dadín Prando
Schares, Gereon
Campero, Carlos Manuel
Venturini, María Cecilia
author_facet Bacigalupe, Diana Raquel
Basso, Walter Ubaldo
Caspe, S. G.
Moré, Gastón Andrés
Lischinsky, L.
Gos, María Laura
Leunda, María Rosa
Campero, Lucía María
Moore, Dadín Prando
Schares, Gereon
Campero, Carlos Manuel
Venturini, María Cecilia
author_sort Bacigalupe, Diana Raquel
title <i>Neospora caninum</i> NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams
title_short <i>Neospora caninum</i> NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams
title_full <i>Neospora caninum</i> NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams
title_fullStr <i>Neospora caninum</i> NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams
title_full_unstemmed <i>Neospora caninum</i> NC-6 Argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams
title_sort <i>neospora caninum</i> nc-6 argentina induces fetopathy in both serologically positive and negative experimentally inoculated pregnant dams
publishDate 2013
url http://sedici.unlp.edu.ar/handle/10915/137194
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