Penduliflorain I: A Cysteine Protease Isolated from Hohenbergia penduliflora (A.Rich.) Mez (Bromeliaceae)

Penduliflorain I, a new plant endopeptidase, was isolated and characterized from Hohenbergia penduliflora. Crude extract was obtained from stems. A partially purified enzyme preparation was obtained by ethanol precipitation. This preparation showed maximum activity between pH 7.5...

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Detalles Bibliográficos
Autores principales:	Pérez, Aurora, Carvajal, Carol, Trejo, Sebastián Alejandro, Figuerero Torres, María José, Martin, María Inés, Lorenzo, José Carlos, Natalucci, Claudia Luisa, Hernández, Martha
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2010
Materias:	Ciencias Exactas Biología Bromeliaceae Hohenbergia penduliflora Chromatography purification Stems Thiol proteases
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/137088
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-137088
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Biología Bromeliaceae Hohenbergia penduliflora Chromatography purification Stems Thiol proteases
spellingShingle	Ciencias Exactas Biología Bromeliaceae Hohenbergia penduliflora Chromatography purification Stems Thiol proteases Pérez, Aurora Carvajal, Carol Trejo, Sebastián Alejandro Figuerero Torres, María José Martin, María Inés Lorenzo, José Carlos Natalucci, Claudia Luisa Hernández, Martha Penduliflorain I: A Cysteine Protease Isolated from <i>Hohenbergia penduliflora</i> (A.Rich.) Mez (<i>Bromeliaceae</i>)
topic_facet	Ciencias Exactas Biología Bromeliaceae Hohenbergia penduliflora Chromatography purification Stems Thiol proteases
description	Penduliflorain I, a new plant endopeptidase, was isolated and characterized from <i>Hohenbergia penduliflora</i>. Crude extract was obtained from stems. A partially purified enzyme preparation was obtained by ethanol precipitation. This preparation showed maximum activity between pH 7.5 and 8.5, was stable at ionic strength (20% decrease in proteolytic activity could be detected after 2 h in 0.4 M sodium chloride solution), and exhibited high thermal stability (inactivation required heating for 20 min at 75 °C). Inhibition and activation assays indicated the cysteine nature of the enzymatic preparation. Penduliflorain I was purified by anion exchange chromatography (Q-Sepharose HP) by FPLC system. Homogeneity was confirmed by mass spectroscopy. Molecular mass of the enzyme was 23 412.847 Da (MALDI-TOF–MS). Kinetic parameters were determined for PFLNA (Kₘ = 0.3227 mM and k<sub>cat</sub> = 4.27 s⁻¹). The N-terminal sequence (AVPQSIDWRDYGAVTTDKNQ) of isolated protease showed considerable similarity to other cysteine proteases obtained from stems or fruits of different <i>Bromeliaceae</i> species.
format	Articulo Articulo
author	Pérez, Aurora Carvajal, Carol Trejo, Sebastián Alejandro Figuerero Torres, María José Martin, María Inés Lorenzo, José Carlos Natalucci, Claudia Luisa Hernández, Martha
author_facet	Pérez, Aurora Carvajal, Carol Trejo, Sebastián Alejandro Figuerero Torres, María José Martin, María Inés Lorenzo, José Carlos Natalucci, Claudia Luisa Hernández, Martha
author_sort	Pérez, Aurora
title	Penduliflorain I: A Cysteine Protease Isolated from <i>Hohenbergia penduliflora</i> (A.Rich.) Mez (<i>Bromeliaceae</i>)
title_short	Penduliflorain I: A Cysteine Protease Isolated from <i>Hohenbergia penduliflora</i> (A.Rich.) Mez (<i>Bromeliaceae</i>)
title_full	Penduliflorain I: A Cysteine Protease Isolated from <i>Hohenbergia penduliflora</i> (A.Rich.) Mez (<i>Bromeliaceae</i>)
title_fullStr	Penduliflorain I: A Cysteine Protease Isolated from <i>Hohenbergia penduliflora</i> (A.Rich.) Mez (<i>Bromeliaceae</i>)
title_full_unstemmed	Penduliflorain I: A Cysteine Protease Isolated from <i>Hohenbergia penduliflora</i> (A.Rich.) Mez (<i>Bromeliaceae</i>)
title_sort	penduliflorain i: a cysteine protease isolated from <i>hohenbergia penduliflora</i> (a.rich.) mez (<i>bromeliaceae</i>)
publishDate	2010
url	http://sedici.unlp.edu.ar/handle/10915/137088
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Penduliflorain I: A Cysteine Protease Isolated from <i>Hohenbergia penduliflora</i> (A.Rich.) Mez (<i>Bromeliaceae</i>)

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