Bacteriomes of the corn leafhopper, Dalbulus maidis (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor Sulcia symbiont: molecular characterization, ultrastructure, and transovarial transmission

In this study, we surveyed the bacteriome-associated microbiota of the corn leafhopper Dalbulus maidis by means of histological, ultrastructural, and molecular analyses. Amplification and sequencing of 16S rDNA genes revealed that the endosymbiont “Candidatus Su...

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Detalles Bibliográficos
Autores principales:	Brentassi, María Eugenia, Franco, Mario Emilio Ernesto, Balatti, Pedro Alberto, Medina, Rocío, Bernabei, Franco, Marino de Remes Lenicov, Ana María
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2017
Materias:	Ciencias Naturales Ciencias Exactas "Candidatus Sulcia muelleri" Bacteriomes Corn stunt Corn leafhopper Obligate endosymbionts
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/136965
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-136965
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Naturales Ciencias Exactas "Candidatus Sulcia muelleri" Bacteriomes Corn stunt Corn leafhopper Obligate endosymbionts
spellingShingle	Ciencias Naturales Ciencias Exactas "Candidatus Sulcia muelleri" Bacteriomes Corn stunt Corn leafhopper Obligate endosymbionts Brentassi, María Eugenia Franco, Mario Emilio Ernesto Balatti, Pedro Alberto Medina, Rocío Bernabei, Franco Marino de Remes Lenicov, Ana María Bacteriomes of the corn leafhopper, <i>Dalbulus maidis</i> (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor <i>Sulcia</i> symbiont: molecular characterization, ultrastructure, and transovarial transmission
topic_facet	Ciencias Naturales Ciencias Exactas "Candidatus Sulcia muelleri" Bacteriomes Corn stunt Corn leafhopper Obligate endosymbionts
description	In this study, we surveyed the bacteriome-associated microbiota of the corn leafhopper <i>Dalbulus maidis</i> by means of histological, ultrastructural, and molecular analyses. Amplification and sequencing of 16S rDNA genes revealed that the endosymbiont “<i>Candidatus</i> Sulcia muelleri” (Phylum Bacteroidetes) resides in bacteriomes of <i>D. maidis</i>. Phylogenetic analysis showed that the sequence was closely allied to others found in representatives of the subfamily Deltocephalinae. We failed to amplify other sequences as “<i>Candidatus</i> Nasuia deltocephalinicola,” a co-primary symbiont frequently associated to deltocephaline leafhoppers. In addition, a metagenetic analysis carried out in order to investigate the presence of other bacteriome-associated bacteria of <i>D. maidis</i> showed that the sequence of <i>Sulcia</i> accounted for 98.56 % of all the sequences. Histological and ultrastructural observations showed that microorganisms harbored in bacteriomes (central syncytium and cytoplasm of uninucleate bacteriocytes) look like others <i>Sulcia</i> described in hemipteran species and they were transovarially transmitted from mother to offspring which is typical of obligate endosymbionts. The only presence of <i>Sulcia</i> in the bacteriomes of <i>D. maidis</i> was discussed.
format	Articulo Articulo
author	Brentassi, María Eugenia Franco, Mario Emilio Ernesto Balatti, Pedro Alberto Medina, Rocío Bernabei, Franco Marino de Remes Lenicov, Ana María
author_facet	Brentassi, María Eugenia Franco, Mario Emilio Ernesto Balatti, Pedro Alberto Medina, Rocío Bernabei, Franco Marino de Remes Lenicov, Ana María
author_sort	Brentassi, María Eugenia
title	Bacteriomes of the corn leafhopper, <i>Dalbulus maidis</i> (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor <i>Sulcia</i> symbiont: molecular characterization, ultrastructure, and transovarial transmission
title_short	Bacteriomes of the corn leafhopper, <i>Dalbulus maidis</i> (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor <i>Sulcia</i> symbiont: molecular characterization, ultrastructure, and transovarial transmission
title_full	Bacteriomes of the corn leafhopper, <i>Dalbulus maidis</i> (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor <i>Sulcia</i> symbiont: molecular characterization, ultrastructure, and transovarial transmission
title_fullStr	Bacteriomes of the corn leafhopper, <i>Dalbulus maidis</i> (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor <i>Sulcia</i> symbiont: molecular characterization, ultrastructure, and transovarial transmission
title_full_unstemmed	Bacteriomes of the corn leafhopper, <i>Dalbulus maidis</i> (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor <i>Sulcia</i> symbiont: molecular characterization, ultrastructure, and transovarial transmission
title_sort	bacteriomes of the corn leafhopper, <i>dalbulus maidis</i> (delong & wolcott, 1923) (insecta, hemiptera, cicadellidae: deltocephalinae) harbor <i>sulcia</i> symbiont: molecular characterization, ultrastructure, and transovarial transmission
publishDate	2017
url	http://sedici.unlp.edu.ar/handle/10915/136965
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Bacteriomes of the corn leafhopper, <i>Dalbulus maidis</i> (DeLong & Wolcott, 1923) (Insecta, Hemiptera, Cicadellidae: Deltocephalinae) harbor <i>Sulcia</i> symbiont: molecular characterization, ultrastructure, and transovarial transmission

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