Validation of reference transcripts in strawberry (<i>Fragaria</i> spp.)

Contemporary methods to assay gene expression depend on a stable set of reference transcripts for accurate quantitation. A lack of well-tested reference genes slows progress in characterizing gene expression in high-value specialty crops. In this study, a set of strawberry (<i>Fragaria</i&g...

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Detalles Bibliográficos
Autores principales: Clancy, Maureen A., Rosli, Hernán Guillermo, Chamala, Srikar, Barbazuk, W. Brad, Civello, Pedro Marcos, Folta, Kevin M.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2013
Materias:
Ciencias Exactas
Ciencias Agrarias
Fragaria
Gene expression
Normalization
Reference genes
Strawberry
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/136836
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-136836
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Ciencias Agrarias
Fragaria
Gene expression
Normalization
Reference genes
Strawberry
spellingShingle Ciencias Exactas
Ciencias Agrarias
Fragaria
Gene expression
Normalization
Reference genes
Strawberry
Clancy, Maureen A.
Rosli, Hernán Guillermo
Chamala, Srikar
Barbazuk, W. Brad
Civello, Pedro Marcos
Folta, Kevin M.
Validation of reference transcripts in strawberry (<i>Fragaria</i> spp.)
topic_facet Ciencias Exactas
Ciencias Agrarias
Fragaria
Gene expression
Normalization
Reference genes
Strawberry
description Contemporary methods to assay gene expression depend on a stable set of reference transcripts for accurate quantitation. A lack of well-tested reference genes slows progress in characterizing gene expression in high-value specialty crops. In this study, a set of strawberry (<i>Fragaria</i> spp.) constitutively expressed reference genes has been identified by merging digital gene expression data with expression profiling. Constitutive reference candidates were validated using quantitative PCR and hybridization. Several transcripts have been identified that show improved stability across tissues relative to traditional reference transcripts. Results are similar between commercial octoploid strawberry and the diploid model. Our findings also show that while some never-before-used references are appropriate for most applications, even the most stable reference transcripts require careful assessment across the diverse tissues and fruit developmental states before being adopted as controls.
format Articulo
Articulo
author Clancy, Maureen A.
Rosli, Hernán Guillermo
Chamala, Srikar
Barbazuk, W. Brad
Civello, Pedro Marcos
Folta, Kevin M.
author_facet Clancy, Maureen A.
Rosli, Hernán Guillermo
Chamala, Srikar
Barbazuk, W. Brad
Civello, Pedro Marcos
Folta, Kevin M.
author_sort Clancy, Maureen A.
title Validation of reference transcripts in strawberry (<i>Fragaria</i> spp.)
title_short Validation of reference transcripts in strawberry (<i>Fragaria</i> spp.)
title_full Validation of reference transcripts in strawberry (<i>Fragaria</i> spp.)
title_fullStr Validation of reference transcripts in strawberry (<i>Fragaria</i> spp.)
title_full_unstemmed Validation of reference transcripts in strawberry (<i>Fragaria</i> spp.)
title_sort validation of reference transcripts in strawberry (<i>fragaria</i> spp.)
publishDate 2013
url http://sedici.unlp.edu.ar/handle/10915/136836
work_keys_str_mv AT clancymaureena validationofreferencetranscriptsinstrawberryifragariaispp
AT roslihernanguillermo validationofreferencetranscriptsinstrawberryifragariaispp
AT chamalasrikar validationofreferencetranscriptsinstrawberryifragariaispp
AT barbazukwbrad validationofreferencetranscriptsinstrawberryifragariaispp
AT civellopedromarcos validationofreferencetranscriptsinstrawberryifragariaispp
AT foltakevinm validationofreferencetranscriptsinstrawberryifragariaispp
bdutipo_str Repositorios
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