An imazethapyr-based herbicide formulation induces genotoxic, biochemical, and individual organizational effects in <i>Leptodactylus latinasus</i> tadpoles (Anura: Leptodactylidae)

Genotoxic, biochemical, and individual organizational effects on <i>Leptodactylus latinasus</i> tadpoles were evaluated after exposure to an imazethapyr (IMZT)-based commercial herbicide formulation, Pivot® H (10.59% IMZT). A determination of the value of the lethal concentration (LC50)...

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Autores principales: Pérez Iglesias, Juan Manuel, Brodeur, Julie C., Larramendy, Marcelo Luis
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
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Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/136695
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id I19-R120-10915-136695
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Naturales
Química
Imazethapyr herbicide formulation
Lethality
Genotoxicity
Oxidative stress
Morphological and behavioral alterations
spellingShingle Ciencias Naturales
Química
Imazethapyr herbicide formulation
Lethality
Genotoxicity
Oxidative stress
Morphological and behavioral alterations
Pérez Iglesias, Juan Manuel
Brodeur, Julie C.
Larramendy, Marcelo Luis
An imazethapyr-based herbicide formulation induces genotoxic, biochemical, and individual organizational effects in <i>Leptodactylus latinasus</i> tadpoles (Anura: Leptodactylidae)
topic_facet Ciencias Naturales
Química
Imazethapyr herbicide formulation
Lethality
Genotoxicity
Oxidative stress
Morphological and behavioral alterations
description Genotoxic, biochemical, and individual organizational effects on <i>Leptodactylus latinasus</i> tadpoles were evaluated after exposure to an imazethapyr (IMZT)-based commercial herbicide formulation, Pivot® H (10.59% IMZT). A determination of the value of the lethal concentration (LC50) was determined as a toxicological endpoint. Alterations in animal behavior and morphological abnormalities as well as cholinesterase (ChE), catalase (CAT), and glutathione S-transferase (GST) activities were employed as individual sublethal endpoints. Micronuclei frequencies (MNs), binucleated cells (BNs), blebbed nuclei (BLs), lobed nuclei (LBs), notched nuclei (NTs), erythroplastids (EPs), and evaluation of DNA strand breaks were employed as genotoxic endpoints. All biomarkers were evaluated after 48 and 96 h of exposure to concentrations of IMZT within 0.07-4.89 mg/L. LC50<sub>96h</sub> values of 1.01 and 0.29 mg/L IMZT were obtained for Gosner stages 25 and 36, respectively. Irregular swimming, diamond body shape, and decreased frequency of keratodonts were detected at both sampling times. Results showed that IMZT increased GST activity and MN frequency at 48 and 96 h of exposure. Other nuclear abnormalities were also observed in the circulating erythrocytes of tadpoles, i.e., NT and BL values after 48 h, and LN, BL, and EP values after 96 h. Finally, results showed that IMZT within 0.07-0.22 mg/L increased the genetic damage index in tadpoles exposed for both exposure times (48 and 96 h). This study is the first to report the sublethal biochemical effects of IMZT in anurans and is also the first report using <i>L. latinasus</i> tadpoles as a bioindicator for ecotoxicological studies.
format Articulo
Articulo
author Pérez Iglesias, Juan Manuel
Brodeur, Julie C.
Larramendy, Marcelo Luis
author_facet Pérez Iglesias, Juan Manuel
Brodeur, Julie C.
Larramendy, Marcelo Luis
author_sort Pérez Iglesias, Juan Manuel
title An imazethapyr-based herbicide formulation induces genotoxic, biochemical, and individual organizational effects in <i>Leptodactylus latinasus</i> tadpoles (Anura: Leptodactylidae)
title_short An imazethapyr-based herbicide formulation induces genotoxic, biochemical, and individual organizational effects in <i>Leptodactylus latinasus</i> tadpoles (Anura: Leptodactylidae)
title_full An imazethapyr-based herbicide formulation induces genotoxic, biochemical, and individual organizational effects in <i>Leptodactylus latinasus</i> tadpoles (Anura: Leptodactylidae)
title_fullStr An imazethapyr-based herbicide formulation induces genotoxic, biochemical, and individual organizational effects in <i>Leptodactylus latinasus</i> tadpoles (Anura: Leptodactylidae)
title_full_unstemmed An imazethapyr-based herbicide formulation induces genotoxic, biochemical, and individual organizational effects in <i>Leptodactylus latinasus</i> tadpoles (Anura: Leptodactylidae)
title_sort imazethapyr-based herbicide formulation induces genotoxic, biochemical, and individual organizational effects in <i>leptodactylus latinasus</i> tadpoles (anura: leptodactylidae)
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/136695
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