The carbon source influences the energetic efficiency of the respiratory chain of N2-fixing Acetobacter diazotrophicus

Acetobacter diazotrophicus is a diazotrophic bacterium that colonizes sugarcane tissues. Glucose is oxidized to gluconate in the periplasm prior to uptake and metabolism. A membrane-bound glucose dehydrogenase quinoenzyme [which contains pyrroloquinoline quinone (PQQ) as the prosthetic group] is inv...

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Detalles Bibliográficos
Autores principales: Luna, María Flavia, Mignone, Carlos Fernando, Boiardi, José Luis
Formato: Articulo
Lenguaje:Inglés
Publicado: 2000
Materias:
Biología
Química
Sugarcane
Respiratory Chain
Hexose
Biomass Yield
Gluconate
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/136614
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-136614
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Química
Sugarcane
Respiratory Chain
Hexose
Biomass Yield
Gluconate
spellingShingle Biología
Química
Sugarcane
Respiratory Chain
Hexose
Biomass Yield
Gluconate
Luna, María Flavia
Mignone, Carlos Fernando
Boiardi, José Luis
The carbon source influences the energetic efficiency of the respiratory chain of N2-fixing Acetobacter diazotrophicus
topic_facet Biología
Química
Sugarcane
Respiratory Chain
Hexose
Biomass Yield
Gluconate
description Acetobacter diazotrophicus is a diazotrophic bacterium that colonizes sugarcane tissues. Glucose is oxidized to gluconate in the periplasm prior to uptake and metabolism. A membrane-bound glucose dehydrogenase quinoenzyme [which contains pyrroloquinoline quinone (PQQ) as the prosthetic group] is involved in that oxidation. Gluconate is oxidized further via the hexose monophosphate pathway and tricarboxylic acid cycle. A. diazotrophicus PAL3 was grown in a chemostat with atmospheric nitrogen as the sole N source provided that the dissolved oxygen was maintained at 1.0–2.0% air saturation. The biomass yields of A. diazotrophicus growing with glucose or gluconate with fixed N were very low compared with other heterotrophic bacteria. The biomass yields under N-fixing conditions were more than 30% less than with ammonium as the N source using gluconate as the carbon source but, surprisingly, were only about 14% less with glucose. The following scheme for the metabolism of A. diazotrophicus through the different pathways emerged: (1) the respiratory chain of this organism had a different efficiency of ATP production in the respiratory chain (P:O ratio) under different culture conditions; and (2) N fixation was one (but not the sole) condition under which a higher P:O ratio was observed. The other condition appears to be the expression of an active PQQ-linked glucose dehydrogenase.
format Articulo
Articulo
author Luna, María Flavia
Mignone, Carlos Fernando
Boiardi, José Luis
author_facet Luna, María Flavia
Mignone, Carlos Fernando
Boiardi, José Luis
author_sort Luna, María Flavia
title The carbon source influences the energetic efficiency of the respiratory chain of N2-fixing Acetobacter diazotrophicus
title_short The carbon source influences the energetic efficiency of the respiratory chain of N2-fixing Acetobacter diazotrophicus
title_full The carbon source influences the energetic efficiency of the respiratory chain of N2-fixing Acetobacter diazotrophicus
title_fullStr The carbon source influences the energetic efficiency of the respiratory chain of N2-fixing Acetobacter diazotrophicus
title_full_unstemmed The carbon source influences the energetic efficiency of the respiratory chain of N2-fixing Acetobacter diazotrophicus
title_sort carbon source influences the energetic efficiency of the respiratory chain of n2-fixing acetobacter diazotrophicus
publishDate 2000
url http://sedici.unlp.edu.ar/handle/10915/136614
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