Expression of p24 gag Protein of Bovine Leukemia Virus in Insect Cells and Its Use in Immunodetection of the Disease

Bovine leukemia is a common retroviral infection of cattle. The disease is characterized by a strong immunological response to several viral proteins, but the antibodies against p24 and gp51 are predominant. In this study, a recombinant baculovirus containing the gag gene p24 was constructed and the...

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Autores principales: Larsen, Alejandra Edith, González, Ester Teresa, Serena, María Soledad, Echeverría, María Gabriela, Mórtola, Eduardo Carlos
Formato: Articulo
Lenguaje:Inglés
Publicado: 2013
Materias:
Veterinaria
Bovine leukemia virus
Immunodetection
Baculovirus system
Recombinant p24 protein
Immunoblotting assay
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/136518
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-136518
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Veterinaria
Bovine leukemia virus
Immunodetection
Baculovirus system
Recombinant p24 protein
Immunoblotting assay
spellingShingle Veterinaria
Bovine leukemia virus
Immunodetection
Baculovirus system
Recombinant p24 protein
Immunoblotting assay
Larsen, Alejandra Edith
González, Ester Teresa
Serena, María Soledad
Echeverría, María Gabriela
Mórtola, Eduardo Carlos
Expression of p24 gag Protein of Bovine Leukemia Virus in Insect Cells and Its Use in Immunodetection of the Disease
topic_facet Veterinaria
Bovine leukemia virus
Immunodetection
Baculovirus system
Recombinant p24 protein
Immunoblotting assay
description Bovine leukemia is a common retroviral infection of cattle. The disease is characterized by a strong immunological response to several viral proteins, but the antibodies against p24 and gp51 are predominant. In this study, a recombinant baculovirus containing the gag gene p24 was constructed and the protein, used as antigen, analyzed by western blot and an indirect in-house rp24-ELISA test. This allowed detecting the presence of antibodies for bovine leukemia virus in a panel of cattle sera. The authentication of the protein expands its potential use for different medical applications, from improved diagnosis of the disease to source of antigens to be included in a subunit vaccine.
format Articulo
Articulo
author Larsen, Alejandra Edith
González, Ester Teresa
Serena, María Soledad
Echeverría, María Gabriela
Mórtola, Eduardo Carlos
author_facet Larsen, Alejandra Edith
González, Ester Teresa
Serena, María Soledad
Echeverría, María Gabriela
Mórtola, Eduardo Carlos
author_sort Larsen, Alejandra Edith
title Expression of p24 gag Protein of Bovine Leukemia Virus in Insect Cells and Its Use in Immunodetection of the Disease
title_short Expression of p24 gag Protein of Bovine Leukemia Virus in Insect Cells and Its Use in Immunodetection of the Disease
title_full Expression of p24 gag Protein of Bovine Leukemia Virus in Insect Cells and Its Use in Immunodetection of the Disease
title_fullStr Expression of p24 gag Protein of Bovine Leukemia Virus in Insect Cells and Its Use in Immunodetection of the Disease
title_full_unstemmed Expression of p24 gag Protein of Bovine Leukemia Virus in Insect Cells and Its Use in Immunodetection of the Disease
title_sort expression of p24 gag protein of bovine leukemia virus in insect cells and its use in immunodetection of the disease
publishDate 2013
url http://sedici.unlp.edu.ar/handle/10915/136518
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