Correlation between fatty acyl composition in neutral and polar lipids and enzyme activities from various tissues of calcium-deficient rats

In this study we investigated the changes induced by feeding rats a calcium-deficient diet (0.5 g Ca/kg diet) during 65 d after weaning. Phospholipase A2, acyl-Co synthetase and FA Δ9-, Δ6-, and Δ5-desaturase activities were also determined. Calcium deficiency evoked a general alteration in the qual...

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Autores principales: Marra, Carlos Alberto, Rimoldi, Omar Jorge, Tacconi de Alaniz, María Josefa
Formato: Articulo
Lenguaje:Inglés
Publicado: 2002
Materias:
Bioquímica
Fatty acyl
Calcium deprivation
Phospholipase A2
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/136409
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-136409
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Fatty acyl
Calcium deprivation
Phospholipase A2
spellingShingle Bioquímica
Fatty acyl
Calcium deprivation
Phospholipase A2
Marra, Carlos Alberto
Rimoldi, Omar Jorge
Tacconi de Alaniz, María Josefa
Correlation between fatty acyl composition in neutral and polar lipids and enzyme activities from various tissues of calcium-deficient rats
topic_facet Bioquímica
Fatty acyl
Calcium deprivation
Phospholipase A2
description In this study we investigated the changes induced by feeding rats a calcium-deficient diet (0.5 g Ca/kg diet) during 65 d after weaning. Phospholipase A2, acyl-Co synthetase and FA Δ9-, Δ6-, and Δ5-desaturase activities were also determined. Calcium deficiency evoked a general alteration in the quality and proportion of the FA chains acylated to neutral and polar lipids from liver, lungs, spleen, brain, kidneys, fat, articular cartilage, erythrocyte ghosts, and plasmas, characterized by an increment of saturated FA and a significant depletion of polyunsaturated acids derived from linoleate and α-linolenate. Several interlipid and lipid/protein relationships were also modified in microsomes from calcium-deprived rats, with a concomitant reduction in the rotational mobility of the probe diphenylhexatriene. Phospholipase A2 and acyl-CoA synthetase activities were also decreased and increased, respectively, in some tissues from calcium-deficient rats, whereas Δ9-, Δ6- and Δ5-desaturases were significantly depressed. We conclude that changes in tissue fatty acyl composition evoked by calcium deprivation are due to alterations in the acylation/deacylation cycles via inhibition of the phospholipase A2. These changes were reflected in the physicochemical properties of the membranes, which in turn inhibits desaturase activities. A possible failure in the transcriptional rate for desaturase-mRNA was also discussed.
format Articulo
Articulo
author Marra, Carlos Alberto
Rimoldi, Omar Jorge
Tacconi de Alaniz, María Josefa
author_facet Marra, Carlos Alberto
Rimoldi, Omar Jorge
Tacconi de Alaniz, María Josefa
author_sort Marra, Carlos Alberto
title Correlation between fatty acyl composition in neutral and polar lipids and enzyme activities from various tissues of calcium-deficient rats
title_short Correlation between fatty acyl composition in neutral and polar lipids and enzyme activities from various tissues of calcium-deficient rats
title_full Correlation between fatty acyl composition in neutral and polar lipids and enzyme activities from various tissues of calcium-deficient rats
title_fullStr Correlation between fatty acyl composition in neutral and polar lipids and enzyme activities from various tissues of calcium-deficient rats
title_full_unstemmed Correlation between fatty acyl composition in neutral and polar lipids and enzyme activities from various tissues of calcium-deficient rats
title_sort correlation between fatty acyl composition in neutral and polar lipids and enzyme activities from various tissues of calcium-deficient rats
publishDate 2002
url http://sedici.unlp.edu.ar/handle/10915/136409
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AT rimoldiomarjorge correlationbetweenfattyacylcompositioninneutralandpolarlipidsandenzymeactivitiesfromvarioustissuesofcalciumdeficientrats
AT tacconidealanizmariajosefa correlationbetweenfattyacylcompositioninneutralandpolarlipidsandenzymeactivitiesfromvarioustissuesofcalciumdeficientrats
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