Adsorption of Rhizobium meliloti to alfalfa roots: dependence on divalent cations and pH

Adsorption of Rhizobium meliloti L5-30 in low numbers to alfalfa (Medicago sativa L.) roots was dependent on the presence of divalent cations, and required neutral pH. Adsorption was proportional to Ca and/or Mg concentrations up to 1.5 mM. Ca was not substituted by Sr, Ba or Mn. Adsorption was abol...

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Detalles Bibliográficos
Autores principales: Caetano Anollés, Gustavo, Lagares, Antonio, Favelukes, Gabriel
Formato: Articulo
Lenguaje:Inglés
Publicado: 1989
Materias:
Química
Bacterial adsorption
Calcium
Magnesium
Medicago sativa
pH
Rhizobium meliloti
Root surface
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/136321
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-136321
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Bacterial adsorption
Calcium
Magnesium
Medicago sativa
pH
Rhizobium meliloti
Root surface
spellingShingle Química
Bacterial adsorption
Calcium
Magnesium
Medicago sativa
pH
Rhizobium meliloti
Root surface
Caetano Anollés, Gustavo
Lagares, Antonio
Favelukes, Gabriel
Adsorption of Rhizobium meliloti to alfalfa roots: dependence on divalent cations and pH
topic_facet Química
Bacterial adsorption
Calcium
Magnesium
Medicago sativa
pH
Rhizobium meliloti
Root surface
description Adsorption of Rhizobium meliloti L5-30 in low numbers to alfalfa (Medicago sativa L.) roots was dependent on the presence of divalent cations, and required neutral pH. Adsorption was proportional to Ca and/or Mg concentrations up to 1.5 mM. Ca was not substituted by Sr, Ba or Mn. Adsorption was abolished and viability decreased at pH≤6. When lowering pH, higher Ca concentrations were required to attain similar adsorption levels, indicating a marked interactive effect between Ca and H ions. Pretreatment of the roots with Ca and low pH did not affect subsequent adsorption of the bacteria. However, Ca pretreatment ofR. meliloti sustained further adsorption at low Ca levels and low pH substantially affected their ability to adsorb. Low pH appears to affect the stability of binding causing desorption of the previously bound bacteria. The presence of saturating concentrations of heterologousR. leguminosarum bv.trifolii A118, did not prevent the expression of divalent cations and pH requirements, as well as their interaction. Our results suggest that rhizobial binding to the root surface already shows the Ca and pH dependence of alfalfa nodulation, which was generally associated to some event prior to rhizobial penetration of root hairs.
format Articulo
Articulo
author Caetano Anollés, Gustavo
Lagares, Antonio
Favelukes, Gabriel
author_facet Caetano Anollés, Gustavo
Lagares, Antonio
Favelukes, Gabriel
author_sort Caetano Anollés, Gustavo
title Adsorption of Rhizobium meliloti to alfalfa roots: dependence on divalent cations and pH
title_short Adsorption of Rhizobium meliloti to alfalfa roots: dependence on divalent cations and pH
title_full Adsorption of Rhizobium meliloti to alfalfa roots: dependence on divalent cations and pH
title_fullStr Adsorption of Rhizobium meliloti to alfalfa roots: dependence on divalent cations and pH
title_full_unstemmed Adsorption of Rhizobium meliloti to alfalfa roots: dependence on divalent cations and pH
title_sort adsorption of rhizobium meliloti to alfalfa roots: dependence on divalent cations and ph
publishDate 1989
url http://sedici.unlp.edu.ar/handle/10915/136321
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AT lagaresantonio adsorptionofrhizobiummelilotitoalfalfarootsdependenceondivalentcationsandph
AT favelukesgabriel adsorptionofrhizobiummelilotitoalfalfarootsdependenceondivalentcationsandph
bdutipo_str Repositorios
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