Diagnosis of Sarcocystis cruzi, Neospora caninum, and Toxoplasma gondii infections in cattle

The aim of the study was to diagnose Sarcocystis sp. infections in cattle and to detect coinfections by Toxoplasma gondii and/or Neospora caninum. Blood, diaphragm, esophagus, and myocardium from 90 beef cattle from Argentina were collected. Histopathological, immunohistochemical, polymerase chain r...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Moré, Gastón Andrés, Basso, Walter Ubaldo, Bacigalupe, Diana Raquel, Venturini, María Cecilia, Venturini, María Lucila
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2008
Materias:	Ciencias Veterinarias Beef Cattle Indirect Fluorescent Antibody Test Adult Cattle Sarcocystis Species Seropositive Animal
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/136315
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-136315
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Veterinarias Beef Cattle Indirect Fluorescent Antibody Test Adult Cattle Sarcocystis Species Seropositive Animal
spellingShingle	Ciencias Veterinarias Beef Cattle Indirect Fluorescent Antibody Test Adult Cattle Sarcocystis Species Seropositive Animal Moré, Gastón Andrés Basso, Walter Ubaldo Bacigalupe, Diana Raquel Venturini, María Cecilia Venturini, María Lucila Diagnosis of Sarcocystis cruzi, Neospora caninum, and Toxoplasma gondii infections in cattle
topic_facet	Ciencias Veterinarias Beef Cattle Indirect Fluorescent Antibody Test Adult Cattle Sarcocystis Species Seropositive Animal
description	The aim of the study was to diagnose Sarcocystis sp. infections in cattle and to detect coinfections by Toxoplasma gondii and/or Neospora caninum. Blood, diaphragm, esophagus, and myocardium from 90 beef cattle from Argentina were collected. Histopathological, immunohistochemical, polymerase chain reaction assays, and direct microscopical examination were carried out. Sarcocysts from myocardium were measured and counted. Indirect fluorescent antibody test (IFAT) for the three protozoans was performed. Sarcocystis cruzi sarcocysts were found in 100% of myocardium samples. Sarcocysts per gram ranged from 8 to 380 with higher values found in adult cattle (p < 0.001). T. gondii and N. caninum were not detected by immunohistochemistry. T. gondii DNA was found in myocardium of 2/20 seropositive animals, while N. caninum DNA was not found. Antibodies against S. cruzi were detected in all samples, those against N. caninum in 73% and against T. gondii in 91% of the samples (IFAT titer ≥25). It is concluded that serology by IFAT is a suitable method to diagnose these protozoan infections due to its specific IgG detection; therefore, IFAT may be a useful tool to evaluate the impact of each protozoan infection in coinfected animals.
format	Articulo Articulo
author	Moré, Gastón Andrés Basso, Walter Ubaldo Bacigalupe, Diana Raquel Venturini, María Cecilia Venturini, María Lucila
author_facet	Moré, Gastón Andrés Basso, Walter Ubaldo Bacigalupe, Diana Raquel Venturini, María Cecilia Venturini, María Lucila
author_sort	Moré, Gastón Andrés
title	Diagnosis of Sarcocystis cruzi, Neospora caninum, and Toxoplasma gondii infections in cattle
title_short	Diagnosis of Sarcocystis cruzi, Neospora caninum, and Toxoplasma gondii infections in cattle
title_full	Diagnosis of Sarcocystis cruzi, Neospora caninum, and Toxoplasma gondii infections in cattle
title_fullStr	Diagnosis of Sarcocystis cruzi, Neospora caninum, and Toxoplasma gondii infections in cattle
title_full_unstemmed	Diagnosis of Sarcocystis cruzi, Neospora caninum, and Toxoplasma gondii infections in cattle
title_sort	diagnosis of sarcocystis cruzi, neospora caninum, and toxoplasma gondii infections in cattle
publishDate	2008
url	http://sedici.unlp.edu.ar/handle/10915/136315
work_keys_str_mv	AT moregastonandres diagnosisofsarcocystiscruzineosporacaninumandtoxoplasmagondiiinfectionsincattle AT bassowalterubaldo diagnosisofsarcocystiscruzineosporacaninumandtoxoplasmagondiiinfectionsincattle AT bacigalupedianaraquel diagnosisofsarcocystiscruzineosporacaninumandtoxoplasmagondiiinfectionsincattle AT venturinimariacecilia diagnosisofsarcocystiscruzineosporacaninumandtoxoplasmagondiiinfectionsincattle AT venturinimarialucila diagnosisofsarcocystiscruzineosporacaninumandtoxoplasmagondiiinfectionsincattle
bdutipo_str	Repositorios
_version_	1764820456655814660

Diagnosis of Sarcocystis cruzi, Neospora caninum, and Toxoplasma gondii infections in cattle

Ejemplares similares