Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirus

The ecdysteroid UDP-glycosyltransferase (egt) gene of <i>Epinotia aporema</i> granulovirus (EpapGV) was cloned sequenced and its biological activity was assessed. It encodes a protein of 446 amino acids. Direct evidence that the cloned gene encodes an active EGT protein was obtained by t...

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Detalles Bibliográficos
Autores principales:	Manzán, María Alejandra, Lozano, Mario Enrique, Sciocco de Cap, Alicia, Ghiringhelli, Pablo Daniel, Romanowski, Víctor
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2002
Materias:	Biología baculoviruses ecdysone glycosyltransferase (egt) EpapGV gene function granuloviruses
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/136119
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-136119
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Biología baculoviruses ecdysone glycosyltransferase (egt) EpapGV gene function granuloviruses
spellingShingle	Biología baculoviruses ecdysone glycosyltransferase (egt) EpapGV gene function granuloviruses Manzán, María Alejandra Lozano, Mario Enrique Sciocco de Cap, Alicia Ghiringhelli, Pablo Daniel Romanowski, Víctor Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirus
topic_facet	Biología baculoviruses ecdysone glycosyltransferase (egt) EpapGV gene function granuloviruses
description	The ecdysteroid UDP-glycosyltransferase (egt) gene of <i>Epinotia aporema</i> granulovirus (EpapGV) was cloned sequenced and its biological activity was assessed. It encodes a protein of 446 amino acids. Direct evidence that the cloned gene encodes an active EGT protein was obtained by transient expression assays in insect cells. The upstream untranslated region of the egt gene exhibits several consensus early promoter elements. Accordingly, the gene is expressed early upon infection of <i>Epinotia aporema</i> larvae and the EGT activity remains high until later times post infection. Sequence analyses indicate the presence of clusters of amino acid residues conserved among all the baculoviral EGTs, although their relation with proper protein folding, ligand binding and catalytic activity remain to be assessed. Phylogenetic trees consistently cluster the granulovirus EGTs separating them clearly from the nucleopolyhedroviruses.
format	Articulo Articulo
author	Manzán, María Alejandra Lozano, Mario Enrique Sciocco de Cap, Alicia Ghiringhelli, Pablo Daniel Romanowski, Víctor
author_facet	Manzán, María Alejandra Lozano, Mario Enrique Sciocco de Cap, Alicia Ghiringhelli, Pablo Daniel Romanowski, Víctor
author_sort	Manzán, María Alejandra
title	Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirus
title_short	Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirus
title_full	Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirus
title_fullStr	Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirus
title_full_unstemmed	Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirus
title_sort	identification and characterization of the ecdysteroid udp-glycosyltransferase gene of <i>epinotia aporema</i> granulovirus
publishDate	2002
url	http://sedici.unlp.edu.ar/handle/10915/136119
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Identification and characterization of the ecdysteroid UDP-glycosyltransferase gene of <i>Epinotia aporema</i> granulovirus

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