A conventional PCR technique to detect <i>Septoria tritici</i> in wheat seeds

A PCR assay was developed for detection of wheat seed naturally contaminated with <i>Septoria tritici</i>. <i>S. tritici</i> specific primers were derived from strict alignment of ITS and α-tubulin sequences of the pathogen. The specificity of four sets of synthesised oligonu...

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Detalles Bibliográficos
Autores principales: Consolo, Verónica Fabiana, Albani, Clara María, Berón, Corina M., Salerno, Graciela Lidia, Cordo, Cristina Alicia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2009
Materias:
Ciencias Agrarias
Ciencias Naturales
Mycosphaerella graminicola
seedborne pathogen
Septoria leaf blotch
wheat
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/136109
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-136109
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Agrarias
Ciencias Naturales
Mycosphaerella graminicola
seedborne pathogen
Septoria leaf blotch
wheat
spellingShingle Ciencias Agrarias
Ciencias Naturales
Mycosphaerella graminicola
seedborne pathogen
Septoria leaf blotch
wheat
Consolo, Verónica Fabiana
Albani, Clara María
Berón, Corina M.
Salerno, Graciela Lidia
Cordo, Cristina Alicia
A conventional PCR technique to detect <i>Septoria tritici</i> in wheat seeds
topic_facet Ciencias Agrarias
Ciencias Naturales
Mycosphaerella graminicola
seedborne pathogen
Septoria leaf blotch
wheat
description A PCR assay was developed for detection of wheat seed naturally contaminated with <i>Septoria tritici</i>. <i>S. tritici</i> specific primers were derived from strict alignment of ITS and α-tubulin sequences of the pathogen. The specificity of four sets of synthesised oligonucleotide pairs (A, B, C and D) were tested using isolates from S. tritici, other selected fungi and wheat seeds. A single DNA fragment was amplified from <i>S. tritici</i> isolates with all primer pairs, whereas no product was generated from other DNA sources. <i>S. tritici</i> was also detected in wheat seed lots collected from plants with variable pycnidial coverage on the upper two leaves. PCR detection of as little as 0.5 pg of <i>S. tritici</i> genomic DNAwas possible. This is the first report on the detection of <i>S. tritici</i> DNA in naturally infested wheat seeds. This PCR based assay is simple, rapid, specific, sensitive and suitable for routine detection of the wheat pathogen in infested wheat seeds.
format Articulo
Articulo
author Consolo, Verónica Fabiana
Albani, Clara María
Berón, Corina M.
Salerno, Graciela Lidia
Cordo, Cristina Alicia
author_facet Consolo, Verónica Fabiana
Albani, Clara María
Berón, Corina M.
Salerno, Graciela Lidia
Cordo, Cristina Alicia
author_sort Consolo, Verónica Fabiana
title A conventional PCR technique to detect <i>Septoria tritici</i> in wheat seeds
title_short A conventional PCR technique to detect <i>Septoria tritici</i> in wheat seeds
title_full A conventional PCR technique to detect <i>Septoria tritici</i> in wheat seeds
title_fullStr A conventional PCR technique to detect <i>Septoria tritici</i> in wheat seeds
title_full_unstemmed A conventional PCR technique to detect <i>Septoria tritici</i> in wheat seeds
title_sort conventional pcr technique to detect <i>septoria tritici</i> in wheat seeds
publishDate 2009
url http://sedici.unlp.edu.ar/handle/10915/136109
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