Glucocorticoid-induced apoptosis in lymphoid organs is associated with a delayed increase in circulating deoxyribonucleic acid

Pathological processes like cancer, chronic inflammation and autoimmune phenomena, all of which involve massive cell death, are associated with significant increases in circulating DNA. In order to clarify whether massive apoptosis occurring under physiological circumstances also causes DNA release...

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Detalles Bibliográficos
Autores principales: Goya, Rodolfo Gustavo, Console-Avegliano, Gloria Miriam, Spinelli, Osvaldo Mateo, Carino, Mónica H., Riccillo, Fernando Luis, Corrons, Félix J.
Formato: Articulo
Lenguaje:Español
Publicado: 2003
Materias:
Medicina
Apoptosis
delayed release
dexamethasone
plasma DNA
spleen
thymocyte
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/136103
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-136103
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Español
topic Medicina
Apoptosis
delayed release
dexamethasone
plasma DNA
spleen
thymocyte
spellingShingle Medicina
Apoptosis
delayed release
dexamethasone
plasma DNA
spleen
thymocyte
Goya, Rodolfo Gustavo
Console-Avegliano, Gloria Miriam
Spinelli, Osvaldo Mateo
Carino, Mónica H.
Riccillo, Fernando Luis
Corrons, Félix J.
Glucocorticoid-induced apoptosis in lymphoid organs is associated with a delayed increase in circulating deoxyribonucleic acid
topic_facet Medicina
Apoptosis
delayed release
dexamethasone
plasma DNA
spleen
thymocyte
description Pathological processes like cancer, chronic inflammation and autoimmune phenomena, all of which involve massive cell death, are associated with significant increases in circulating DNA. In order to clarify whether massive apoptosis occurring under physiological circumstances also causes DNA release into the circulation, we correlated the time-course of dexamethasone-induced intra thymic cell apoptosis with plasma DNA dynamics in rats. Animals were given 10 mg/l dexamethasone in their drinking water for up to 7 days. Sequential plasma samples were obtained during the treatment and DNA was quantitated by a micro fluorometric assay. Thymus and spleen weight as well as apoptotic cell levels were assessed at different times. Seven days of glucocorticoid treatment reduced thymic and spleen mass by 82 and 31%, respectively. Intra thymic apoptosis was maximal 24 h after the beginning of glucocorticoid treatment, declining markedly by 48 h. Very little apoptosis was observed in the spleen. Plasma DNA increased steadily during the first 4 days of glucocorticoid treatment (11.8 ± 1.2 μg/ml on day 0; 24.2 ± 1.6 μg/ml on day 4) beginning to decline afterward. Thymectomy but not splenectomy, drastically reduced the glucocorticoid-induced increase in plasma DNA. It is concluded that hormone-induced massive intra thymic cell death is followed by a delayed release of nucleosomal DNA into the circulation.
format Articulo
Articulo
author Goya, Rodolfo Gustavo
Console-Avegliano, Gloria Miriam
Spinelli, Osvaldo Mateo
Carino, Mónica H.
Riccillo, Fernando Luis
Corrons, Félix J.
author_facet Goya, Rodolfo Gustavo
Console-Avegliano, Gloria Miriam
Spinelli, Osvaldo Mateo
Carino, Mónica H.
Riccillo, Fernando Luis
Corrons, Félix J.
author_sort Goya, Rodolfo Gustavo
title Glucocorticoid-induced apoptosis in lymphoid organs is associated with a delayed increase in circulating deoxyribonucleic acid
title_short Glucocorticoid-induced apoptosis in lymphoid organs is associated with a delayed increase in circulating deoxyribonucleic acid
title_full Glucocorticoid-induced apoptosis in lymphoid organs is associated with a delayed increase in circulating deoxyribonucleic acid
title_fullStr Glucocorticoid-induced apoptosis in lymphoid organs is associated with a delayed increase in circulating deoxyribonucleic acid
title_full_unstemmed Glucocorticoid-induced apoptosis in lymphoid organs is associated with a delayed increase in circulating deoxyribonucleic acid
title_sort glucocorticoid-induced apoptosis in lymphoid organs is associated with a delayed increase in circulating deoxyribonucleic acid
publishDate 2003
url http://sedici.unlp.edu.ar/handle/10915/136103
work_keys_str_mv AT goyarodolfogustavo glucocorticoidinducedapoptosisinlymphoidorgansisassociatedwithadelayedincreaseincirculatingdeoxyribonucleicacid
AT consoleaveglianogloriamiriam glucocorticoidinducedapoptosisinlymphoidorgansisassociatedwithadelayedincreaseincirculatingdeoxyribonucleicacid
AT spinelliosvaldomateo glucocorticoidinducedapoptosisinlymphoidorgansisassociatedwithadelayedincreaseincirculatingdeoxyribonucleicacid
AT carinomonicah glucocorticoidinducedapoptosisinlymphoidorgansisassociatedwithadelayedincreaseincirculatingdeoxyribonucleicacid
AT riccillofernandoluis glucocorticoidinducedapoptosisinlymphoidorgansisassociatedwithadelayedincreaseincirculatingdeoxyribonucleicacid
AT corronsfelixj glucocorticoidinducedapoptosisinlymphoidorgansisassociatedwithadelayedincreaseincirculatingdeoxyribonucleicacid
bdutipo_str Repositorios
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