Role of S-layer proteins in the biosorption capacity of lead by <i>Lactobacillus kefir</i>
The role of S-layer proteins (SLP) on the Pb²⁺ sequestrant capacity by <i>Lactobacillus kefir</i> CIDCA 8348 and JCM 5818 was investigated. Cultures in the stationary phase were treated with proteinase K. A dot blot assay was carried out to assess the removal of SLP. Strains with and wit...
| Autores principales: | , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2015
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/135854 |
| Aporte de: |
| id |
I19-R120-10915-135854 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Exactas Biología S-layer proteins Lactobacillus kefir Biosorption Lead |
| spellingShingle |
Ciencias Exactas Biología S-layer proteins Lactobacillus kefir Biosorption Lead Gerbino, Oscar Esteban Carasi, Paula Araujo Andrade, Cuauhtémoc Tymczyszyn, Emma Elizabeth Gómez-Zavaglia, Andrea Role of S-layer proteins in the biosorption capacity of lead by <i>Lactobacillus kefir</i> |
| topic_facet |
Ciencias Exactas Biología S-layer proteins Lactobacillus kefir Biosorption Lead |
| description |
The role of S-layer proteins (SLP) on the Pb²⁺ sequestrant capacity by <i>Lactobacillus kefir</i> CIDCA 8348 and JCM 5818 was investigated. Cultures in the stationary phase were treated with proteinase K. A dot blot assay was carried out to assess the removal of SLP. Strains with and without SLP were exposed to 0–0.5 mM Pb(NO₃)₂. The maximum binding capacity (q<sub>max</sub>) and the affinity coefficient (b) were calculated using the Langmuir equation. The structural effect of Pb²⁺ on microorganisms with and without SLP was determined using Raman spectroscopy. The bacterial interaction with Pb²⁺ led to a broadening in the phosphate bands (1,300–1,200 cm⁻¹ region) and strong alterations on amide and carboxylate-related bands (νCOO⁻ as and νCOO⁻ s). Microorganisms without SLP removed higher percentages of Pb²⁺ and had higher q<sub>max</sub> than those bearing SLP. Isolated SLP had much lower q<sub>max</sub> and also removed lower percentages of Pb²⁺ than the corresponding whole microorganisms. The hydrofobicity of both strains dramatically dropped when removing SLP. When bearing SLP, strains do not expose a large amount of charged groups on their surfaces, thus making less efficient the Pb²⁺ removal. On the contrary, the extremely low hydrofobicity of microorganisms without SLP (and consequently, their higher capacity to remove Pb²⁺) can be explained on the basis of a greater exposure of charged chemical groups for the interaction with Pb²⁺. The viability of bacteria without SLP was not significantly lower than that of bacteria bearing SLP. However, microorganisms without SLP were more prone to the detrimental effect of Pb²⁺, thus suggesting that SLP acts as a protective rather than as a sequestrant layer. |
| format |
Articulo Articulo |
| author |
Gerbino, Oscar Esteban Carasi, Paula Araujo Andrade, Cuauhtémoc Tymczyszyn, Emma Elizabeth Gómez-Zavaglia, Andrea |
| author_facet |
Gerbino, Oscar Esteban Carasi, Paula Araujo Andrade, Cuauhtémoc Tymczyszyn, Emma Elizabeth Gómez-Zavaglia, Andrea |
| author_sort |
Gerbino, Oscar Esteban |
| title |
Role of S-layer proteins in the biosorption capacity of lead by <i>Lactobacillus kefir</i> |
| title_short |
Role of S-layer proteins in the biosorption capacity of lead by <i>Lactobacillus kefir</i> |
| title_full |
Role of S-layer proteins in the biosorption capacity of lead by <i>Lactobacillus kefir</i> |
| title_fullStr |
Role of S-layer proteins in the biosorption capacity of lead by <i>Lactobacillus kefir</i> |
| title_full_unstemmed |
Role of S-layer proteins in the biosorption capacity of lead by <i>Lactobacillus kefir</i> |
| title_sort |
role of s-layer proteins in the biosorption capacity of lead by <i>lactobacillus kefir</i> |
| publishDate |
2015 |
| url |
http://sedici.unlp.edu.ar/handle/10915/135854 |
| work_keys_str_mv |
AT gerbinooscaresteban roleofslayerproteinsinthebiosorptioncapacityofleadbyilactobacilluskefiri AT carasipaula roleofslayerproteinsinthebiosorptioncapacityofleadbyilactobacilluskefiri AT araujoandradecuauhtemoc roleofslayerproteinsinthebiosorptioncapacityofleadbyilactobacilluskefiri AT tymczyszynemmaelizabeth roleofslayerproteinsinthebiosorptioncapacityofleadbyilactobacilluskefiri AT gomezzavagliaandrea roleofslayerproteinsinthebiosorptioncapacityofleadbyilactobacilluskefiri |
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Repositorios |
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