Dataset for Cyclic di-GMP regulates type three secretion system and virulence in <i>Bordetella bronchiseptica</i>

The second messenger c-di-GMP is a ubiquitous molecule in bacteria that regulates diverse phenotypes. Among them, motility and biofilm formation are the most studied. Furthermore, c-di-GMP has been suggested to regulate virulence factors, therefore making it important for pathogenesis. Previously we...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Gutierrez, María de la Paz, Wong, Ting, Damron, Frederick, Fernández, Julieta, Sisti, Federico
Formato: Conjunto de datos
Lenguaje:Inglés
Publicado: 2022
Materias:
Ciencias Exactas
https://purl.org/becyt/ford/1.6
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/135429
https://doi.org/10.35537/10915/135429
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-135429
record_format dspace
spelling I19-R120-10915-1354292023-04-27T15:09:32Z http://sedici.unlp.edu.ar/handle/10915/135429 https://doi.org/10.35537/10915/135429 Dataset for Cyclic di-GMP regulates type three secretion system and virulence in <i>Bordetella bronchiseptica</i> Gutierrez, María de la Paz Wong, Ting Damron, Frederick Fernández, Julieta Sisti, Federico 2022-05-02T16:58:49Z 2021 en Ciencias Exactas https://purl.org/becyt/ford/1.6 The second messenger c-di-GMP is a ubiquitous molecule in bacteria that regulates diverse phenotypes. Among them, motility and biofilm formation are the most studied. Furthermore, c-di-GMP has been suggested to regulate virulence factors, therefore making it important for pathogenesis. Previously we described that c-di-GMP regulates biofilm formation and swimming motility in Bordetella bronchiseptica. Here we present a multi-omic approach on B. bronchiseptica strains expressing different cytoplasmic c-di-GMP levels, including RNA-seq and shotgun proteomics with label-free quantification. We detected 64 proteins significantly up- or down-regulated in either low or high c-di-GMP levels and 358 genes differentially expressed between high c-di-GMP levels and the wild type strain. Among them we found stress-related proteins, nitrogen metabolism enzymes, phage-related genes and virulence factors. Interestingly, we observed that a virulence factor like the type three secretion system (TTSS) was regulated by c-di-GMP. B. bronchiseptica with high c-di-GMP levels showed significantly lower levels of TTSS components like Bsp22, BopN and Bcr4. These findings were confirmed by independent methods as q-RT PCR and western blot. Higher intracellular levels of c-di-GMP correlated with impaired capacity to induce cytotoxicity in a eukaryotic cell in vitro and with attenuated virulence in a murine model. This work presents data that will support the role that the second messenger c-di-GMP plays in the pathogenesis of Bordetellae. Fil: Fernández, Julieta. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Instituto de Biotecnología y Biología Molecular; Argentina. Fil: Gutierrez, María de la Paz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular, Argentina. Fil: Gutierrez, María de la Paz. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Sisti, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Facultad de Ciencias Exactas Consejo Nacional de Investigaciones Científicas y Técnicas Conjunto de datos Conjunto de datos http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/vnd.ms-excel
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
https://purl.org/becyt/ford/1.6
spellingShingle Ciencias Exactas
https://purl.org/becyt/ford/1.6
Gutierrez, María de la Paz
Wong, Ting
Damron, Frederick
Fernández, Julieta
Sisti, Federico
Dataset for Cyclic di-GMP regulates type three secretion system and virulence in <i>Bordetella bronchiseptica</i>
topic_facet Ciencias Exactas
https://purl.org/becyt/ford/1.6
description The second messenger c-di-GMP is a ubiquitous molecule in bacteria that regulates diverse phenotypes. Among them, motility and biofilm formation are the most studied. Furthermore, c-di-GMP has been suggested to regulate virulence factors, therefore making it important for pathogenesis. Previously we described that c-di-GMP regulates biofilm formation and swimming motility in Bordetella bronchiseptica. Here we present a multi-omic approach on B. bronchiseptica strains expressing different cytoplasmic c-di-GMP levels, including RNA-seq and shotgun proteomics with label-free quantification. We detected 64 proteins significantly up- or down-regulated in either low or high c-di-GMP levels and 358 genes differentially expressed between high c-di-GMP levels and the wild type strain. Among them we found stress-related proteins, nitrogen metabolism enzymes, phage-related genes and virulence factors. Interestingly, we observed that a virulence factor like the type three secretion system (TTSS) was regulated by c-di-GMP. B. bronchiseptica with high c-di-GMP levels showed significantly lower levels of TTSS components like Bsp22, BopN and Bcr4. These findings were confirmed by independent methods as q-RT PCR and western blot. Higher intracellular levels of c-di-GMP correlated with impaired capacity to induce cytotoxicity in a eukaryotic cell in vitro and with attenuated virulence in a murine model. This work presents data that will support the role that the second messenger c-di-GMP plays in the pathogenesis of Bordetellae.
format Conjunto de datos
Conjunto de datos
author Gutierrez, María de la Paz
Wong, Ting
Damron, Frederick
Fernández, Julieta
Sisti, Federico
author_facet Gutierrez, María de la Paz
Wong, Ting
Damron, Frederick
Fernández, Julieta
Sisti, Federico
author_sort Gutierrez, María de la Paz
title Dataset for Cyclic di-GMP regulates type three secretion system and virulence in <i>Bordetella bronchiseptica</i>
title_short Dataset for Cyclic di-GMP regulates type three secretion system and virulence in <i>Bordetella bronchiseptica</i>
title_full Dataset for Cyclic di-GMP regulates type three secretion system and virulence in <i>Bordetella bronchiseptica</i>
title_fullStr Dataset for Cyclic di-GMP regulates type three secretion system and virulence in <i>Bordetella bronchiseptica</i>
title_full_unstemmed Dataset for Cyclic di-GMP regulates type three secretion system and virulence in <i>Bordetella bronchiseptica</i>
title_sort dataset for cyclic di-gmp regulates type three secretion system and virulence in <i>bordetella bronchiseptica</i>
publishDate 2022
url http://sedici.unlp.edu.ar/handle/10915/135429
https://doi.org/10.35537/10915/135429
work_keys_str_mv AT gutierrezmariadelapaz datasetforcyclicdigmpregulatestypethreesecretionsystemandvirulenceinibordetellabronchisepticai
AT wongting datasetforcyclicdigmpregulatestypethreesecretionsystemandvirulenceinibordetellabronchisepticai
AT damronfrederick datasetforcyclicdigmpregulatestypethreesecretionsystemandvirulenceinibordetellabronchisepticai
AT fernandezjulieta datasetforcyclicdigmpregulatestypethreesecretionsystemandvirulenceinibordetellabronchisepticai
AT sistifederico datasetforcyclicdigmpregulatestypethreesecretionsystemandvirulenceinibordetellabronchisepticai
_version_ 1765659849714040832

Ejemplares similares