The neuropeptide RhoprCCHamide2 inhibits serotonin-stimulated transcellular Na⁺ transport across the anterior midgut of the vector of Chagas disease, <i>Rhodnius prolixus</i>
<i>Rhodnius prolixus</i> is a blood-feeding insect vector of <i>Trypanosoma cruzi</i>, a protozoan parasite that causes Chagas disease. During each blood meal, the animals ingest large volumes of blood, that may be up to 12 times the unfed body mass. These blood meals impose...
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| Autores principales: | , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
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2021
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/135420 |
| Aporte de: |
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I19-R120-10915-135420 |
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dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Exactas Biología CCHamide2 Crop Ion transport Osmoregulation Rhodnius prolixus |
| spellingShingle |
Ciencias Exactas Biología CCHamide2 Crop Ion transport Osmoregulation Rhodnius prolixus Capriotti, Natalia Gioino, Paula Ons, Sheila Ianowski, Juan P. The neuropeptide RhoprCCHamide2 inhibits serotonin-stimulated transcellular Na⁺ transport across the anterior midgut of the vector of Chagas disease, <i>Rhodnius prolixus</i> |
| topic_facet |
Ciencias Exactas Biología CCHamide2 Crop Ion transport Osmoregulation Rhodnius prolixus |
| description |
<i>Rhodnius prolixus</i> is a blood-feeding insect vector of <i>Trypanosoma cruzi</i>, a protozoan parasite that causes Chagas disease. During each blood meal, the animals ingest large volumes of blood, that may be up to 12 times the unfed body mass. These blood meals impose a significant osmotic stress for the animals due to the hyposmotic condition of the ingested blood compared with the insect's hemolymph. Thus the insect undergoes a massive postprandial diuresis that allows for the excretion of the plasma fraction of the blood in less than two hours. Diuresis is performed by the excretory system, consisting of the Malpighian tubules and gut, under the control of diuretic and anti-diuretic factors. We investigated the ion transport machinery triggered by stimulation with the diuretic factor serotonin in the anterior midgut (i.e. crop) and the effect of the diuretic modulator RhoprCCHamide2. Ussing chamber assays revealed that serotonin-stimulated increase in transepithelial short-circuit current (I<sub>sc</sub>) was more sensitive to the blockage with amiloride than 5-N-ethyl-N-isopropyl amiloride (EIPA), suggesting the involvement of Na⁺ channels. Incubation in Na⁺-free, but not Cl⁻-free saline, blocked the effect of serotonin on I<sub>sc</sub>. Moreover, treatment with Na⁺-K⁺-2Cl⁻ cotransporter (NKCC) and Na⁺-Cl⁻ cotransporter (NCC) blockers had no effect on fluid secretion but was blocked by amiloride. Blockage of Na⁺/K⁺-ATPase with ouabain inhibited I<sub>sc</sub> but the H⁺-ATPase inhibitor bafilomycin had no effect. The neuropeptide RhoprCCHamide2 diminished serotonin-stimulated I<sub>sc</sub> across the crop. The results suggest that Na⁺ undergoes active transport via an apical amiloride-sensitive Na⁺ channel and a basolateral ouabain-sensitive Na⁺/K⁺-ATPase, while Cl⁻ is transported through a passive paracellular pathway. |
| format |
Articulo Articulo |
| author |
Capriotti, Natalia Gioino, Paula Ons, Sheila Ianowski, Juan P. |
| author_facet |
Capriotti, Natalia Gioino, Paula Ons, Sheila Ianowski, Juan P. |
| author_sort |
Capriotti, Natalia |
| title |
The neuropeptide RhoprCCHamide2 inhibits serotonin-stimulated transcellular Na⁺ transport across the anterior midgut of the vector of Chagas disease, <i>Rhodnius prolixus</i> |
| title_short |
The neuropeptide RhoprCCHamide2 inhibits serotonin-stimulated transcellular Na⁺ transport across the anterior midgut of the vector of Chagas disease, <i>Rhodnius prolixus</i> |
| title_full |
The neuropeptide RhoprCCHamide2 inhibits serotonin-stimulated transcellular Na⁺ transport across the anterior midgut of the vector of Chagas disease, <i>Rhodnius prolixus</i> |
| title_fullStr |
The neuropeptide RhoprCCHamide2 inhibits serotonin-stimulated transcellular Na⁺ transport across the anterior midgut of the vector of Chagas disease, <i>Rhodnius prolixus</i> |
| title_full_unstemmed |
The neuropeptide RhoprCCHamide2 inhibits serotonin-stimulated transcellular Na⁺ transport across the anterior midgut of the vector of Chagas disease, <i>Rhodnius prolixus</i> |
| title_sort |
neuropeptide rhoprcchamide2 inhibits serotonin-stimulated transcellular na⁺ transport across the anterior midgut of the vector of chagas disease, <i>rhodnius prolixus</i> |
| publishDate |
2021 |
| url |
http://sedici.unlp.edu.ar/handle/10915/135420 |
| work_keys_str_mv |
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Repositorios |
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1764820455352434688 |