Lactobacillus delbrueckii subsp. lactis (strain CIDCA 133) stimulates murine macrophages infected with Citrobacter rodentium

Citrobacter rodentium is a specific murine enteropathogen which causes diarrheal disease characterized by colonic hyperplasia and intestinal inflammation. Recruitment of neutrophils and macrophages constitute a key step to control the infection. Since modulation of the activity of professional phago...

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Autores principales: Hugo, Ayelén, Rolny, Ivanna Sabrina, Romanin, David Emmanuel, Pérez, Pablo Fernando
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Química
Citrobacter rodentium
Lactobacilli
Macrophages
Phagocytosis
Probiotics
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/135186
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-135186
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Citrobacter rodentium
Lactobacilli
Macrophages
Phagocytosis
Probiotics
spellingShingle Química
Citrobacter rodentium
Lactobacilli
Macrophages
Phagocytosis
Probiotics
Hugo, Ayelén
Rolny, Ivanna Sabrina
Romanin, David Emmanuel
Pérez, Pablo Fernando
Lactobacillus delbrueckii subsp. lactis (strain CIDCA 133) stimulates murine macrophages infected with Citrobacter rodentium
topic_facet Química
Citrobacter rodentium
Lactobacilli
Macrophages
Phagocytosis
Probiotics
description Citrobacter rodentium is a specific murine enteropathogen which causes diarrheal disease characterized by colonic hyperplasia and intestinal inflammation. Recruitment of neutrophils and macrophages constitute a key step to control the infection. Since modulation of the activity of professional phagocytic cells could contribute to improve host´s defences against C. rodentium, we investigated the effect of Lactobacillus delbrueckii subsp. lactis (strain CIDCA 133) on the interaction between murine macrophages (RAW 264.7) and C. rodentium. Phagocytosis, surface molecules and inducible nitric oxide synthase (iNOs) expression were determined by flow cytometry. Reactive oxygen species (ROS) were assessed by fluorescence microscopy. The presence of lactobacilli increased phagocytosis of C. rodentium whereas C. rodentium had no effect on lactobacilli internalization. Survival of internalized C. rodentium diminished when strain CIDCA 133 was present. CD-86, MHCII, iNOs expression and nitrite production were increased when C. rodentium and lactobacilli were present even though strain CIDCA 133 alone had no effect. Strain CIDCA 133 led to a strong induction of ROS activity which was not modified by C. rodentium. Lactobacillus delbrueckii subsp. lactis (strain CIDCA 133) is able to increase the activation of murine macrophages infected with C. rodentium. The sole presence of lactobacilli is enough to modify some stimulation markers (e.g. ROS induction) whereas other markers require the presence of both bacteria; thus, indicating a synergistic effect.
format Articulo
Articulo
author Hugo, Ayelén
Rolny, Ivanna Sabrina
Romanin, David Emmanuel
Pérez, Pablo Fernando
author_facet Hugo, Ayelén
Rolny, Ivanna Sabrina
Romanin, David Emmanuel
Pérez, Pablo Fernando
author_sort Hugo, Ayelén
title Lactobacillus delbrueckii subsp. lactis (strain CIDCA 133) stimulates murine macrophages infected with Citrobacter rodentium
title_short Lactobacillus delbrueckii subsp. lactis (strain CIDCA 133) stimulates murine macrophages infected with Citrobacter rodentium
title_full Lactobacillus delbrueckii subsp. lactis (strain CIDCA 133) stimulates murine macrophages infected with Citrobacter rodentium
title_fullStr Lactobacillus delbrueckii subsp. lactis (strain CIDCA 133) stimulates murine macrophages infected with Citrobacter rodentium
title_full_unstemmed Lactobacillus delbrueckii subsp. lactis (strain CIDCA 133) stimulates murine macrophages infected with Citrobacter rodentium
title_sort lactobacillus delbrueckii subsp. lactis (strain cidca 133) stimulates murine macrophages infected with citrobacter rodentium
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/135186
work_keys_str_mv AT hugoayelen lactobacillusdelbrueckiisubsplactisstraincidca133stimulatesmurinemacrophagesinfectedwithcitrobacterrodentium
AT rolnyivannasabrina lactobacillusdelbrueckiisubsplactisstraincidca133stimulatesmurinemacrophagesinfectedwithcitrobacterrodentium
AT romanindavidemmanuel lactobacillusdelbrueckiisubsplactisstraincidca133stimulatesmurinemacrophagesinfectedwithcitrobacterrodentium
AT perezpablofernando lactobacillusdelbrueckiisubsplactisstraincidca133stimulatesmurinemacrophagesinfectedwithcitrobacterrodentium
bdutipo_str Repositorios
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