SARS-CoV-2 codon usage bias downregulates host expressed genes with similar codon usage

Severe acute respiratory syndrome is quickly spreading throughout the world and was declared as a pandemic by the World Health Organisation (WHO). The pathogenic agent is a new coronavirus (SARS-CoV-2) that infects pulmonary cells with great effectiveness. In this study we focus on the codon composi...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Alonso, Andrés M., Diambra, Luis Aníbal
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Biología
SARS-CoV-2
Codon usage bias
Codon optimality
Translational control
Pathogeny
Vaccine design
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/135183
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-135183
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
SARS-CoV-2
Codon usage bias
Codon optimality
Translational control
Pathogeny
Vaccine design
spellingShingle Biología
SARS-CoV-2
Codon usage bias
Codon optimality
Translational control
Pathogeny
Vaccine design
Alonso, Andrés M.
Diambra, Luis Aníbal
SARS-CoV-2 codon usage bias downregulates host expressed genes with similar codon usage
topic_facet Biología
SARS-CoV-2
Codon usage bias
Codon optimality
Translational control
Pathogeny
Vaccine design
description Severe acute respiratory syndrome is quickly spreading throughout the world and was declared as a pandemic by the World Health Organisation (WHO). The pathogenic agent is a new coronavirus (SARS-CoV-2) that infects pulmonary cells with great effectiveness. In this study we focus on the codon composition for the viral proteins synthesis and its relationship with the proteins synthesis of the host. Our analysis reveals that SARS-CoV-2 preferred codons have poor representation of G or C nucleotides in the third position, a characteristic which could conduct to an unbalance in the tRNAs pools of the infected cells with serious implications in host protein synthesis. By integrating this observation with proteomic data from infected cells, we observe a reduced translation rate of host proteins associated with highly expressed genes, and that they share the codon usage bias of the virus. The functional analysis of these genes suggests that this mechanism of epistasis contributes to understand some deleterious collateral effect as result of the viral replication. In this manner, our finding contribute to the understanding of the SARS-CoV-2 pathogeny and could be useful for the design of a vaccine based on the live attenuated strategy.
format Articulo
Articulo
author Alonso, Andrés M.
Diambra, Luis Aníbal
author_facet Alonso, Andrés M.
Diambra, Luis Aníbal
author_sort Alonso, Andrés M.
title SARS-CoV-2 codon usage bias downregulates host expressed genes with similar codon usage
title_short SARS-CoV-2 codon usage bias downregulates host expressed genes with similar codon usage
title_full SARS-CoV-2 codon usage bias downregulates host expressed genes with similar codon usage
title_fullStr SARS-CoV-2 codon usage bias downregulates host expressed genes with similar codon usage
title_full_unstemmed SARS-CoV-2 codon usage bias downregulates host expressed genes with similar codon usage
title_sort sars-cov-2 codon usage bias downregulates host expressed genes with similar codon usage
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/135183
work_keys_str_mv AT alonsoandresm sarscov2codonusagebiasdownregulateshostexpressedgeneswithsimilarcodonusage
AT diambraluisanibal sarscov2codonusagebiasdownregulateshostexpressedgeneswithsimilarcodonusage
bdutipo_str Repositorios
_version_ 1764820456439808002

Ejemplares similares