First Study of Different Insect Cells to Triatoma Virus Infection

The use of viruses for biological control is a new option to be considered. The family Dicistroviridae, which affects only invertebrates, is one of the families that have been proposed for this purpose. The Triatoma virus (TrV), a member of this family, affects triatomine transmitters of Chagas dise...

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Autores principales: Susevich, María Laura, Martí, Gerardo Aníbal, Metz, Germán Ernesto, Echeverría, María Gabriela
Formato: Articulo
Lenguaje:Inglés
Publicado: 2015
Materias:
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/135174
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id I19-R120-10915-135174
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Insect Cell Line
Paralysis Virus
Black Queen Cell Virus
Blind Passage
Taura Syndrome Virus
spellingShingle Biología
Insect Cell Line
Paralysis Virus
Black Queen Cell Virus
Blind Passage
Taura Syndrome Virus
Susevich, María Laura
Martí, Gerardo Aníbal
Metz, Germán Ernesto
Echeverría, María Gabriela
First Study of Different Insect Cells to Triatoma Virus Infection
topic_facet Biología
Insect Cell Line
Paralysis Virus
Black Queen Cell Virus
Blind Passage
Taura Syndrome Virus
description The use of viruses for biological control is a new option to be considered. The family Dicistroviridae, which affects only invertebrates, is one of the families that have been proposed for this purpose. The Triatoma virus (TrV), a member of this family, affects triatomine transmitters of Chagas disease, which is endemic in Latin America but also expanding its worldwide distribution. To this end, we attempted virus replication in Diptera, Aedes albopictus (clone C6/36) and Lepidoptera Spodoptera frugiperda (SF9, SF21) and High Five (H5) cell lines. The methodologies used were transfection process, direct inoculation (purified virus), and inoculation of purified virus with trypsin. Results were confirmed by SDS-PAGE, Western blotting, RT-PCR, electron microscopy, and immunofluorescence. According to the results obtained, further analysis of susceptibility/infection of H5 cells to TrV required to be studied.
format Articulo
Articulo
author Susevich, María Laura
Martí, Gerardo Aníbal
Metz, Germán Ernesto
Echeverría, María Gabriela
author_facet Susevich, María Laura
Martí, Gerardo Aníbal
Metz, Germán Ernesto
Echeverría, María Gabriela
author_sort Susevich, María Laura
title First Study of Different Insect Cells to Triatoma Virus Infection
title_short First Study of Different Insect Cells to Triatoma Virus Infection
title_full First Study of Different Insect Cells to Triatoma Virus Infection
title_fullStr First Study of Different Insect Cells to Triatoma Virus Infection
title_full_unstemmed First Study of Different Insect Cells to Triatoma Virus Infection
title_sort first study of different insect cells to triatoma virus infection
publishDate 2015
url http://sedici.unlp.edu.ar/handle/10915/135174
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