Photothermal therapy with silver nanoplates in HeLa cells studied by <i>in situ</i> fluorescence microscopy

Photothermal therapy (PTT) is a noninvasive treatment for cancer relying on the incorporation of NIR-light absorbing nanomaterials into cells, which upon illumination release heat causing thermally induced cell death. We prove that irradiation of aqueous suspensions of poly(vinylpyrrolidone)-coated...

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Detalles Bibliográficos
Autores principales: Rivas Aiello, María Belén, Azcárate, Julio César, Zelaya, Eugenia, Gara, Pedro David, Bosio, Gabriela Natalia, Gensch, Thomas, Mártire, Daniel Osvaldo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2021
Materias:
Química
Física
photothermal therapy
silver nanoplates
cell
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/135035
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-135035
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Física
photothermal therapy
silver nanoplates
cell
spellingShingle Química
Física
photothermal therapy
silver nanoplates
cell
Rivas Aiello, María Belén
Azcárate, Julio César
Zelaya, Eugenia
Gara, Pedro David
Bosio, Gabriela Natalia
Gensch, Thomas
Mártire, Daniel Osvaldo
Photothermal therapy with silver nanoplates in HeLa cells studied by <i>in situ</i> fluorescence microscopy
topic_facet Química
Física
photothermal therapy
silver nanoplates
cell
description Photothermal therapy (PTT) is a noninvasive treatment for cancer relying on the incorporation of NIR-light absorbing nanomaterials into cells, which upon illumination release heat causing thermally induced cell death. We prove that irradiation of aqueous suspensions of poly(vinylpyrrolidone)-coated silver nanoplates (PVPAgNP) or PVPAgNP in HeLa cells with red or NIR lasers causes a sizeable photothermal effect, which in cells can be visualized with the temperature sensing fluorophore Rhodamine B (RhB) using spinning disk confocal fluorescence microscopy or fluorescence lifetime imaging. Upon red-light irradiation of cells that were incubated with both, RhB and PVPAgNP at concentrations with no adverse effects on cell viability, a substantial heat release is detected. Initiation of cell death by photothermal effect is observed by positive signals of fluorescent markers for early and late apoptosis. Surprisingly, a new nanomaterial-assisted cell killing mode is operating when PVPAgNP-loaded HeLa cells are excited with moderate powers of fs-pulsed NIR light. Small roundish areas are generated with bright and fast (<1 ns) decaying emission, which expand fast and destroy the whole cell in seconds. This characteristic emission is assigned to efficient optical breakdown initiation around the strongly absorbing PVPAgNP leading to plasma formation that spreads fast through the cell.
format Articulo
Articulo
author Rivas Aiello, María Belén
Azcárate, Julio César
Zelaya, Eugenia
Gara, Pedro David
Bosio, Gabriela Natalia
Gensch, Thomas
Mártire, Daniel Osvaldo
author_facet Rivas Aiello, María Belén
Azcárate, Julio César
Zelaya, Eugenia
Gara, Pedro David
Bosio, Gabriela Natalia
Gensch, Thomas
Mártire, Daniel Osvaldo
author_sort Rivas Aiello, María Belén
title Photothermal therapy with silver nanoplates in HeLa cells studied by <i>in situ</i> fluorescence microscopy
title_short Photothermal therapy with silver nanoplates in HeLa cells studied by <i>in situ</i> fluorescence microscopy
title_full Photothermal therapy with silver nanoplates in HeLa cells studied by <i>in situ</i> fluorescence microscopy
title_fullStr Photothermal therapy with silver nanoplates in HeLa cells studied by <i>in situ</i> fluorescence microscopy
title_full_unstemmed Photothermal therapy with silver nanoplates in HeLa cells studied by <i>in situ</i> fluorescence microscopy
title_sort photothermal therapy with silver nanoplates in hela cells studied by <i>in situ</i> fluorescence microscopy
publishDate 2021
url http://sedici.unlp.edu.ar/handle/10915/135035
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bdutipo_str Repositorios
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