Effect of Bacillus cereus exocellular factors on human intestinal epithelial cells

To gain insight on the biological effects of the exocellular factors produced by Bacillus cereus, culture filtrate supernatants of different strains were coincubated with differentiated Caco-2 cells. Exocellular factors were able to detach enterocyte-like cells from the substratum after 1 h of incub...

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Autores principales: Minnaard, Jessica, Humen, Martín Andrés, Pérez, Pablo Fernando
Formato: Articulo
Lenguaje:Inglés
Publicado: 2001
Materias:
Biología
Bacillus
Exocellular factors
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/134985
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-134985
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Bacillus
Exocellular factors
spellingShingle Biología
Bacillus
Exocellular factors
Minnaard, Jessica
Humen, Martín Andrés
Pérez, Pablo Fernando
Effect of Bacillus cereus exocellular factors on human intestinal epithelial cells
topic_facet Biología
Bacillus
Exocellular factors
description To gain insight on the biological effects of the exocellular factors produced by Bacillus cereus, culture filtrate supernatants of different strains were coincubated with differentiated Caco-2 cells. Exocellular factors were able to detach enterocyte-like cells from the substratum after 1 h of incubation. In addition, microvilli effacing and dramatic changes on the cellular surface of enterocytes were found after incubation periods as short as 20 min. Since cell detachment was not inhibited by fetal calf serum, thiol activated cholesterol-binding cytolysin, cereolysin O, does not seem to be involved. Also, translocation of phosphatidylserine from the inner to the outer leaflets of the plasma membrane was demonstrated by using fluorescein isothiocyanate (FITC)-Annexin V. In contrast to the high capability of detaching Caco-2 cells shown by all the strains under study, the mitochondrial dehydrogenase activity was lowered by culture filtrate supernatants in a strain-dependent manner. For strain M2, the decrease in dehydrogenase activity was already evident after 30 min of incubation. Production of biologically active factors depends on the growth phase, and maximal activity was found in late exponential-early stationary phases. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of concentrated exocellular factors showed a very complex scenery supporting the multifactorial character of the biological activity of B. cereus.
format Articulo
Articulo
author Minnaard, Jessica
Humen, Martín Andrés
Pérez, Pablo Fernando
author_facet Minnaard, Jessica
Humen, Martín Andrés
Pérez, Pablo Fernando
author_sort Minnaard, Jessica
title Effect of Bacillus cereus exocellular factors on human intestinal epithelial cells
title_short Effect of Bacillus cereus exocellular factors on human intestinal epithelial cells
title_full Effect of Bacillus cereus exocellular factors on human intestinal epithelial cells
title_fullStr Effect of Bacillus cereus exocellular factors on human intestinal epithelial cells
title_full_unstemmed Effect of Bacillus cereus exocellular factors on human intestinal epithelial cells
title_sort effect of bacillus cereus exocellular factors on human intestinal epithelial cells
publishDate 2001
url http://sedici.unlp.edu.ar/handle/10915/134985
work_keys_str_mv AT minnaardjessica effectofbacilluscereusexocellularfactorsonhumanintestinalepithelialcells
AT humenmartinandres effectofbacilluscereusexocellularfactorsonhumanintestinalepithelialcells
AT perezpablofernando effectofbacilluscereusexocellularfactorsonhumanintestinalepithelialcells
bdutipo_str Repositorios
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