Isolation, characterization and evaluation of mosquitocidal activity of Lysinibacillus strains obtained from Culex pipiens larvae

Three strains—C107, C207, and C307—of spore-forming Gram-positive motile rod-shaped bacteria were isolated from dead larvae of Culex pipiens in La Plata city, Argentina. The three bacterial strains have different phenotypic and molecular characteristics. A comparative analysis of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Tranchida, María Cecilia, Riccillo, Pablo Miguel, Micieli, María Victoria, García, Juan José, Rodriguero, Marcela Silvina
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2011
Materias:	Ciencias Naturales Mosquito larvae Culex pipiens Lysinibacillus sphaericus Biological control 16S rRNA gene
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/134704
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-134704
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Naturales Mosquito larvae Culex pipiens Lysinibacillus sphaericus Biological control 16S rRNA gene
spellingShingle	Ciencias Naturales Mosquito larvae Culex pipiens Lysinibacillus sphaericus Biological control 16S rRNA gene Tranchida, María Cecilia Riccillo, Pablo Miguel Micieli, María Victoria García, Juan José Rodriguero, Marcela Silvina Isolation, characterization and evaluation of mosquitocidal activity of <i>Lysinibacillus</i> strains obtained from <i>Culex pipiens</i> larvae
topic_facet	Ciencias Naturales Mosquito larvae Culex pipiens Lysinibacillus sphaericus Biological control 16S rRNA gene
description	Three strains—C107, C207, and C307—of spore-forming Gram-positive motile rod-shaped bacteria were isolated from dead larvae of <i>Culex pipiens</i> in La Plata city, Argentina. The three bacterial strains have different phenotypic and molecular characteristics. A comparative analysis of their 16S rRNA gene sequences and phylogenetic analysis demonstrated that isolates C107 and C207 were related to reference strains of <i>Lysinibacillus sphaericus</i>, while C307 was related to <i>Bacillus licheniformis</i>. The cytomorphology, biochemical characterization, and phylogenetic relatedness corroborated these respective group assignments. The isolated bacterial strain exhibited the same PCR amplified pattern for the <i>binA</i>, <i>binB</i>, and <i>mtx</i> genes as did the reference strains used. These bacterial strains presented different pathogenic actions among the following mosquito species tested: <i>Culex pipiens</i>, <i>Aedes aegypti</i>, <i>Culex dolosus</i>, <i>Culex apicinus</i>, <i>Ochlerotatus albifasciatus</i>, and <i>Anopheles albitarsis</i>. Only isolates C107 and C207 exhibited mosquitocidal activity. <i>Culex pipiens</i> was the species most susceptible to C107 (LC₅₀, 4 × 10⁴ spores/ml), while <i>O. albifasciatus</i> was most susceptible to C207 (LC₅₀, 3.4 × 10⁶ spores/ml).
format	Articulo Articulo
author	Tranchida, María Cecilia Riccillo, Pablo Miguel Micieli, María Victoria García, Juan José Rodriguero, Marcela Silvina
author_facet	Tranchida, María Cecilia Riccillo, Pablo Miguel Micieli, María Victoria García, Juan José Rodriguero, Marcela Silvina
author_sort	Tranchida, María Cecilia
title	Isolation, characterization and evaluation of mosquitocidal activity of <i>Lysinibacillus</i> strains obtained from <i>Culex pipiens</i> larvae
title_short	Isolation, characterization and evaluation of mosquitocidal activity of <i>Lysinibacillus</i> strains obtained from <i>Culex pipiens</i> larvae
title_full	Isolation, characterization and evaluation of mosquitocidal activity of <i>Lysinibacillus</i> strains obtained from <i>Culex pipiens</i> larvae
title_fullStr	Isolation, characterization and evaluation of mosquitocidal activity of <i>Lysinibacillus</i> strains obtained from <i>Culex pipiens</i> larvae
title_full_unstemmed	Isolation, characterization and evaluation of mosquitocidal activity of <i>Lysinibacillus</i> strains obtained from <i>Culex pipiens</i> larvae
title_sort	isolation, characterization and evaluation of mosquitocidal activity of <i>lysinibacillus</i> strains obtained from <i>culex pipiens</i> larvae
publishDate	2011
url	http://sedici.unlp.edu.ar/handle/10915/134704
work_keys_str_mv	AT tranchidamariacecilia isolationcharacterizationandevaluationofmosquitocidalactivityofilysinibacillusistrainsobtainedfromiculexpipiensilarvae AT riccillopablomiguel isolationcharacterizationandevaluationofmosquitocidalactivityofilysinibacillusistrainsobtainedfromiculexpipiensilarvae AT micielimariavictoria isolationcharacterizationandevaluationofmosquitocidalactivityofilysinibacillusistrainsobtainedfromiculexpipiensilarvae AT garciajuanjose isolationcharacterizationandevaluationofmosquitocidalactivityofilysinibacillusistrainsobtainedfromiculexpipiensilarvae AT rodrigueromarcelasilvina isolationcharacterizationandevaluationofmosquitocidalactivityofilysinibacillusistrainsobtainedfromiculexpipiensilarvae
bdutipo_str	Repositorios
_version_	1764820455324123137

Isolation, characterization and evaluation of mosquitocidal activity of <i>Lysinibacillus</i> strains obtained from <i>Culex pipiens</i> larvae

Ejemplares similares