DNA content and expression of cell cycle proteins in caterpillar nuclei from fetal human cardiac myocytes

Caterpillar nuclei (CN) are characterized by their peculiar morphology, with chromatin distributed in clusters and running along the longitudinal axis of the nucleus. They can be observed in normal hearts of fetuses as well as in hearts of children and adults with rheumatic heart disease. This study...

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Autores principales: García Rivello, Hernán Jorge, Cabeza Meckert, Patricia M., Gallo, Amelia, Palacios, Luis, Laguens, Rubén P.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2002
Materias:
Medicina
Caterpillar nuclei
Cyclin B1
Fetal heart
Nuclear ploidy
PCNA
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/134685
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-134685
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Medicina
Caterpillar nuclei
Cyclin B1
Fetal heart
Nuclear ploidy
PCNA
spellingShingle Medicina
Caterpillar nuclei
Cyclin B1
Fetal heart
Nuclear ploidy
PCNA
García Rivello, Hernán Jorge
Cabeza Meckert, Patricia M.
Gallo, Amelia
Palacios, Luis
Laguens, Rubén P.
DNA content and expression of cell cycle proteins in caterpillar nuclei from fetal human cardiac myocytes
topic_facet Medicina
Caterpillar nuclei
Cyclin B1
Fetal heart
Nuclear ploidy
PCNA
description Caterpillar nuclei (CN) are characterized by their peculiar morphology, with chromatin distributed in clusters and running along the longitudinal axis of the nucleus. They can be observed in normal hearts of fetuses as well as in hearts of children and adults with rheumatic heart disease. This study has demonstrated by means of ploidy studies with digital image analysis that in the fetal heart (20.5±1.8 weeks) the CN (diploid = 5.6±8.4%; tetraploid = 46.2±24.2%; hypertetraploid = 46.9±26.3%) present higher DNA content than non-caterpillar myocyte nuclei (diploid = 89.4±6.2%; tetraploid = 10.0±4.1%; hypertetraploid = 1.5±1.3%) (P=0.000001, 0.00013, and 0.000038, respectively). Expression of proliferation cell nuclear antigen (PCNA; 30.6±11.7% in CN and 13.4±7.3% in non-caterpillar myocyte nuclei; P=0.0115) and cyclin B1 (2.8±3.8% and 12.6±15.6%, respectively; P=n.s.) was also positive in these nuclei. In conclusion, these results suggest that there exists a relationship between CN morphology and myocyte replication phenomena.
format Articulo
Articulo
author García Rivello, Hernán Jorge
Cabeza Meckert, Patricia M.
Gallo, Amelia
Palacios, Luis
Laguens, Rubén P.
author_facet García Rivello, Hernán Jorge
Cabeza Meckert, Patricia M.
Gallo, Amelia
Palacios, Luis
Laguens, Rubén P.
author_sort García Rivello, Hernán Jorge
title DNA content and expression of cell cycle proteins in caterpillar nuclei from fetal human cardiac myocytes
title_short DNA content and expression of cell cycle proteins in caterpillar nuclei from fetal human cardiac myocytes
title_full DNA content and expression of cell cycle proteins in caterpillar nuclei from fetal human cardiac myocytes
title_fullStr DNA content and expression of cell cycle proteins in caterpillar nuclei from fetal human cardiac myocytes
title_full_unstemmed DNA content and expression of cell cycle proteins in caterpillar nuclei from fetal human cardiac myocytes
title_sort dna content and expression of cell cycle proteins in caterpillar nuclei from fetal human cardiac myocytes
publishDate 2002
url http://sedici.unlp.edu.ar/handle/10915/134685
work_keys_str_mv AT garciarivellohernanjorge dnacontentandexpressionofcellcycleproteinsincaterpillarnucleifromfetalhumancardiacmyocytes
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bdutipo_str Repositorios
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