Structural Properties of Macrodontain I, a Cysteine Protease from Pseudananas macrodontes (Morr.) Harms (Bromeliaceae)

The primary structure of macrodontain I, a peptidase from Pseudananas macrodontes fruits, was determined using Edman's degradation. The enzyme is a non-glycosylated peptidase composed by 213 amino acids with a calculated molecular weight of 23,486.18 Da, pI value 6.99, and a molar extinction co...

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Autores principales: Errasti, María Eugenia, Natalucci, Claudia Luisa, Caffini, Néstor Oscar, Rotelli, Alejandra Ester, Brullo, Adriana, Maras, Bruno, Trejo, Sebastián Alejandro, López, Laura María Isabel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Biología
Amino acid sequence
Anti-inflammatory activity
Bromeliaceae
Cysteine peptidase
Macrodontain I
Plant endopeptidase
Pseudananas macrodontes
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/134086
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-134086
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Amino acid sequence
Anti-inflammatory activity
Bromeliaceae
Cysteine peptidase
Macrodontain I
Plant endopeptidase
Pseudananas macrodontes
spellingShingle Biología
Amino acid sequence
Anti-inflammatory activity
Bromeliaceae
Cysteine peptidase
Macrodontain I
Plant endopeptidase
Pseudananas macrodontes
Errasti, María Eugenia
Natalucci, Claudia Luisa
Caffini, Néstor Oscar
Rotelli, Alejandra Ester
Brullo, Adriana
Maras, Bruno
Trejo, Sebastián Alejandro
López, Laura María Isabel
Structural Properties of Macrodontain I, a Cysteine Protease from Pseudananas macrodontes (Morr.) Harms (Bromeliaceae)
topic_facet Biología
Amino acid sequence
Anti-inflammatory activity
Bromeliaceae
Cysteine peptidase
Macrodontain I
Plant endopeptidase
Pseudananas macrodontes
description The primary structure of macrodontain I, a peptidase from Pseudananas macrodontes fruits, was determined using Edman's degradation. The enzyme is a non-glycosylated peptidase composed by 213 amino acids with a calculated molecular weight of 23,486.18 Da, pI value 6.99, and a molar extinction coefficient at 280 nm of 61,685 M<sup>-1</sup> cm<sup>-1</sup>. The alignment of the sequence of macrodontain I with those cysteine peptidases from species belonging to the family Bromeliaceae showed the highest identity degree (87.74%) against fruit bromelain. A remarkable fact is that all these peptidase sequences show two Met contiguous residues (Met121 and 122) and the nonapeptide VPQSIDWRD located in the mature N-terminal region. Residues Cys26 and His159, which constitute the catalytic dyad in all cysteine peptidases, as well as active site residues Gln20 and Asn176, characteristic of Clan C1A, are conserved in macrodontain I. The 3-D model suggests that the enzyme belongs to the α + β class of proteins, with two disulfide bridges (Cys23-Cys63 and Cys57-Cys96) in the α domain, while the β domain is stabilized by another disulfide bridge (Cys153-Cys201). Further, we were able to establish that the cysteine peptidases from P. macrodontes are involved in the anti-inflammatory activity.
format Articulo
Articulo
author Errasti, María Eugenia
Natalucci, Claudia Luisa
Caffini, Néstor Oscar
Rotelli, Alejandra Ester
Brullo, Adriana
Maras, Bruno
Trejo, Sebastián Alejandro
López, Laura María Isabel
author_facet Errasti, María Eugenia
Natalucci, Claudia Luisa
Caffini, Néstor Oscar
Rotelli, Alejandra Ester
Brullo, Adriana
Maras, Bruno
Trejo, Sebastián Alejandro
López, Laura María Isabel
author_sort Errasti, María Eugenia
title Structural Properties of Macrodontain I, a Cysteine Protease from Pseudananas macrodontes (Morr.) Harms (Bromeliaceae)
title_short Structural Properties of Macrodontain I, a Cysteine Protease from Pseudananas macrodontes (Morr.) Harms (Bromeliaceae)
title_full Structural Properties of Macrodontain I, a Cysteine Protease from Pseudananas macrodontes (Morr.) Harms (Bromeliaceae)
title_fullStr Structural Properties of Macrodontain I, a Cysteine Protease from Pseudananas macrodontes (Morr.) Harms (Bromeliaceae)
title_full_unstemmed Structural Properties of Macrodontain I, a Cysteine Protease from Pseudananas macrodontes (Morr.) Harms (Bromeliaceae)
title_sort structural properties of macrodontain i, a cysteine protease from pseudananas macrodontes (morr.) harms (bromeliaceae)
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/134086
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