Purification and Characterization of Four New Cysteine Endopeptidases From Fruits of <i>Bromelia pinguin</i> L. Grown in Cuba
<i>Bromelia pinguin</i> L. is a plant broadly distributed in Central America and Caribbean islands. The fruits have been used in traditional medicine as anthelmintic, probably owed to the presence of a mixture of cysteine endopeptidases, initially termed pinguinain. This work deals with...
| Autores principales: | , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2008
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/133375 |
| Aporte de: |
| id |
I19-R120-10915-133375 |
|---|---|
| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Exactas Biología Bromelia pinguin L. Bromeliaceae Plant cysteine endopeptidases Pinguinain |
| spellingShingle |
Ciencias Exactas Biología Bromelia pinguin L. Bromeliaceae Plant cysteine endopeptidases Pinguinain Abreu Payrol, Juan Obregón, Walter David Trejo, Sebastián Alejandro Caffini, Néstor Oscar Purification and Characterization of Four New Cysteine Endopeptidases From Fruits of <i>Bromelia pinguin</i> L. Grown in Cuba |
| topic_facet |
Ciencias Exactas Biología Bromelia pinguin L. Bromeliaceae Plant cysteine endopeptidases Pinguinain |
| description |
<i>Bromelia pinguin</i> L. is a plant broadly distributed in Central America and Caribbean islands. The fruits have been used in traditional medicine as anthelmintic, probably owed to the presence of a mixture of cysteine endopeptidases, initially termed pinguinain. This work deals with the purification and characterization of the four main components of that mixture, two of them showing acid pI and the other two alkaline pI. Molecular masses (SDS-PAGE and MALDI-TOF), N-terminal sequence and the reactivity and kinetic parameters versus synthetic substrates (p-nitrophenyl-N-α-CBZ-amino acid esters, PFLNA, Z-Arg-Arg-p-NA, and Z-Phe-Arg-p-NA) of the studied peptidases are given, as well as the N-terminal sequences of the enzymes and the homology degree with other plant endopeptidases. |
| format |
Articulo Articulo |
| author |
Abreu Payrol, Juan Obregón, Walter David Trejo, Sebastián Alejandro Caffini, Néstor Oscar |
| author_facet |
Abreu Payrol, Juan Obregón, Walter David Trejo, Sebastián Alejandro Caffini, Néstor Oscar |
| author_sort |
Abreu Payrol, Juan |
| title |
Purification and Characterization of Four New Cysteine Endopeptidases From Fruits of <i>Bromelia pinguin</i> L. Grown in Cuba |
| title_short |
Purification and Characterization of Four New Cysteine Endopeptidases From Fruits of <i>Bromelia pinguin</i> L. Grown in Cuba |
| title_full |
Purification and Characterization of Four New Cysteine Endopeptidases From Fruits of <i>Bromelia pinguin</i> L. Grown in Cuba |
| title_fullStr |
Purification and Characterization of Four New Cysteine Endopeptidases From Fruits of <i>Bromelia pinguin</i> L. Grown in Cuba |
| title_full_unstemmed |
Purification and Characterization of Four New Cysteine Endopeptidases From Fruits of <i>Bromelia pinguin</i> L. Grown in Cuba |
| title_sort |
purification and characterization of four new cysteine endopeptidases from fruits of <i>bromelia pinguin</i> l. grown in cuba |
| publishDate |
2008 |
| url |
http://sedici.unlp.edu.ar/handle/10915/133375 |
| work_keys_str_mv |
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Repositorios |
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1764820454519865344 |