Screening of Bile Resistance and Bile Precipitation in Lactic Acid Bacteria and Bifidobacteria

A modification of the ecometric method was developed for a rapid screening of bile resistance in lactic acid bacteria and bifidobacteria. Validation of the MEM bile assay (modified ecometric method) was performed comparing the bile resistance index (RI<sub>bile</sub>) and the bile resist...

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Detalles Bibliográficos
Autores principales: Kociubinski, Guillermo Luis, Pérez, Pablo Fernando, De Antoni, Graciela Liliana
Formato: Articulo
Lenguaje:Inglés
Publicado: 1999
Materias:
Ciencias Exactas
Biología
bile resistance
lactic acid bacteria
bifidobacteria
ecometric method
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/133325
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-133325
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Biología
bile resistance
lactic acid bacteria
bifidobacteria
ecometric method
spellingShingle Ciencias Exactas
Biología
bile resistance
lactic acid bacteria
bifidobacteria
ecometric method
Kociubinski, Guillermo Luis
Pérez, Pablo Fernando
De Antoni, Graciela Liliana
Screening of Bile Resistance and Bile Precipitation in Lactic Acid Bacteria and Bifidobacteria
topic_facet Ciencias Exactas
Biología
bile resistance
lactic acid bacteria
bifidobacteria
ecometric method
description A modification of the ecometric method was developed for a rapid screening of bile resistance in lactic acid bacteria and bifidobacteria. Validation of the MEM bile assay (modified ecometric method) was performed comparing the bile resistance index (RI<sub>bile</sub>) and the bile resistance ratio (R%). Most <i>Bifidobacterium</i> strains assayed were bile sensitive (83.3%), while only 62.1% of lactobacilli showed that behavior. Some bifidobacterial strains (55.6%) showed a crystalline precipitate when grown on solid medium supplemented with 0.5% ox bile. The crystalline structures produced by <i>B. pseudolongum</i> CIDCA 531 were isolated and analyzed by optical and scanning electron microscopy, thin-layer chromatography, melting point, and specific cholesterol reactions. Those studies confirmed the presence of cholesterol in these crystalline structures. On the other hand, none of the lactobacilli and streptococci studied had the ability to produce crystalline precipitates.
format Articulo
Articulo
author Kociubinski, Guillermo Luis
Pérez, Pablo Fernando
De Antoni, Graciela Liliana
author_facet Kociubinski, Guillermo Luis
Pérez, Pablo Fernando
De Antoni, Graciela Liliana
author_sort Kociubinski, Guillermo Luis
title Screening of Bile Resistance and Bile Precipitation in Lactic Acid Bacteria and Bifidobacteria
title_short Screening of Bile Resistance and Bile Precipitation in Lactic Acid Bacteria and Bifidobacteria
title_full Screening of Bile Resistance and Bile Precipitation in Lactic Acid Bacteria and Bifidobacteria
title_fullStr Screening of Bile Resistance and Bile Precipitation in Lactic Acid Bacteria and Bifidobacteria
title_full_unstemmed Screening of Bile Resistance and Bile Precipitation in Lactic Acid Bacteria and Bifidobacteria
title_sort screening of bile resistance and bile precipitation in lactic acid bacteria and bifidobacteria
publishDate 1999
url http://sedici.unlp.edu.ar/handle/10915/133325
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