Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina

Fusarium graminearum is the primary causal agent of Fusarium head blight of wheat in Argentina. This disease affects yield losses and quality of grains, reducing the wheat end-use, also causing mycotoxin contamination. In this study, the genetic variability and deoxynivalenol (DON) potential/product...

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Autores principales: Ortega, Leonel Maximiliano, Dinolfo, María Inés, Astoreca, Andrea Luciana, Alberione, Enrique Javier, Stenglein, Sebastián Alberto, Alconada Magliano, Teresa María
Formato: Articulo
Lenguaje:Inglés
Publicado: 2015
Materias:
Química
Fusarium graminearum
Wheat
Genetic variability
Deoxynivalenol
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/133323
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-133323
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Fusarium graminearum
Wheat
Genetic variability
Deoxynivalenol
spellingShingle Química
Fusarium graminearum
Wheat
Genetic variability
Deoxynivalenol
Ortega, Leonel Maximiliano
Dinolfo, María Inés
Astoreca, Andrea Luciana
Alberione, Enrique Javier
Stenglein, Sebastián Alberto
Alconada Magliano, Teresa María
Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina
topic_facet Química
Fusarium graminearum
Wheat
Genetic variability
Deoxynivalenol
description Fusarium graminearum is the primary causal agent of Fusarium head blight of wheat in Argentina. This disease affects yield losses and quality of grains, reducing the wheat end-use, also causing mycotoxin contamination. In this study, the genetic variability and deoxynivalenol (DON) potential/production of F. graminearum sensu stricto (s.s.) isolates obtained from wheat samples of the 2009, 2010, and 2011 growing seasons from a single location in Argentina were evaluated. The genetic variability detected using inter-simple sequence repeat (ISSR) was analyzed in relation to the in vitro deoxynivalenol production, the main monitored and quantified mycotoxin according to the current regulations for the international marketing of cereals. Of the 68 F. graminearum s.s. isolates obtained in this study, 95 % showed a different banding pattern with ISSR molecular markers and a high variability was detected within the population. However, no clustering was found in relation with year or DON production. All isolates amplify for the DON-related gene and a high variability in DON production was observed among the isolates, with production values between non-producers and 1741 μg/g. The results suggest that the F. graminearum s.s. population varies significantly in both genetic structure and toxin production in a limited sampled area.
format Articulo
Articulo
author Ortega, Leonel Maximiliano
Dinolfo, María Inés
Astoreca, Andrea Luciana
Alberione, Enrique Javier
Stenglein, Sebastián Alberto
Alconada Magliano, Teresa María
author_facet Ortega, Leonel Maximiliano
Dinolfo, María Inés
Astoreca, Andrea Luciana
Alberione, Enrique Javier
Stenglein, Sebastián Alberto
Alconada Magliano, Teresa María
author_sort Ortega, Leonel Maximiliano
title Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina
title_short Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina
title_full Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina
title_fullStr Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina
title_full_unstemmed Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina
title_sort molecular and mycotoxin characterization of fusarium graminearum isolates obtained from wheat at a single field in argentina
publishDate 2015
url http://sedici.unlp.edu.ar/handle/10915/133323
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