Fast determination of harmala alkaloids in edible algae by capillary electrophoresis mass spectrometry

The use of algae as a foodstuff is rapidly expanding worldwide from the East Asian countries, where they are also used for medical care. Harmala alkaloids (HAlk) are a family of bioactive compounds found in the extracts of some plants, including wakame (<i>Undaria pinnatifida</i>), an ed...

Descripción completa

Detalles Bibliográficos
Autores principales: Tascon, Marcos, Benavente, Fernando, Sanz-Nebot, Victoria M., Gagliardi, Leonardo Gabriel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2015
Materias:
Ciencias Exactas
Química
Capillary electrophoresis
Mass spectrometry
MS–MS
Undaria pinnatifida
Harmala alkaloids
Validation
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/133026
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-133026
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Química
Capillary electrophoresis
Mass spectrometry
MS–MS
Undaria pinnatifida
Harmala alkaloids
Validation
spellingShingle Ciencias Exactas
Química
Capillary electrophoresis
Mass spectrometry
MS–MS
Undaria pinnatifida
Harmala alkaloids
Validation
Tascon, Marcos
Benavente, Fernando
Sanz-Nebot, Victoria M.
Gagliardi, Leonardo Gabriel
Fast determination of harmala alkaloids in edible algae by capillary electrophoresis mass spectrometry
topic_facet Ciencias Exactas
Química
Capillary electrophoresis
Mass spectrometry
MS–MS
Undaria pinnatifida
Harmala alkaloids
Validation
description The use of algae as a foodstuff is rapidly expanding worldwide from the East Asian countries, where they are also used for medical care. Harmala alkaloids (HAlk) are a family of bioactive compounds found in the extracts of some plants, including wakame (<i>Undaria pinnatifida</i>), an edible marine invasive algae. HAlks are based on a characteristic βcarboline structure with at least one amino ionizable group. In this work, we report the successful separation of a mixture of six HAlks (harmine, harmaline, harmol, harmalol, harmane, and norharmane) by capillary electrophoresis ion-trap mass spectrometry (CE-IT-MS) in less than 8 min. Optimum separation in fused-silica capillaries and detection sensitivity in positive-ion mode were achieved using a background electrolyte (BGE) with 25 mmol L⁻¹ ammonium acetate (pH 7.8) and 10 % (v/v) methanol, and a sheath liquid with 60:40 (v/v) isopropanol–water and 0.05 % (v/v) formic acid. The separation method was validated in terms of linearity, limits of detection and quantification, repeatability, and reproducibility. Later, a sample pretreatment was carefully optimized to determine HAlks in commercial wakame samples with excellent recovery and repeatability. For the complex wakame extracts, the MS–MS fragmentation patterns of the different HAlks were useful to ensure a reliable identification. The complete procedure was validated using the standardaddition calibration method, determining matrix effects on the studied compounds. Harmalol, harmine, and harmaline were naturally present in the samples and were quantified at very low concentrations, ranging from 7 to 24 μg kg⁻¹ dry algae.
format Articulo
Articulo
author Tascon, Marcos
Benavente, Fernando
Sanz-Nebot, Victoria M.
Gagliardi, Leonardo Gabriel
author_facet Tascon, Marcos
Benavente, Fernando
Sanz-Nebot, Victoria M.
Gagliardi, Leonardo Gabriel
author_sort Tascon, Marcos
title Fast determination of harmala alkaloids in edible algae by capillary electrophoresis mass spectrometry
title_short Fast determination of harmala alkaloids in edible algae by capillary electrophoresis mass spectrometry
title_full Fast determination of harmala alkaloids in edible algae by capillary electrophoresis mass spectrometry
title_fullStr Fast determination of harmala alkaloids in edible algae by capillary electrophoresis mass spectrometry
title_full_unstemmed Fast determination of harmala alkaloids in edible algae by capillary electrophoresis mass spectrometry
title_sort fast determination of harmala alkaloids in edible algae by capillary electrophoresis mass spectrometry
publishDate 2015
url http://sedici.unlp.edu.ar/handle/10915/133026
work_keys_str_mv AT tasconmarcos fastdeterminationofharmalaalkaloidsinediblealgaebycapillaryelectrophoresismassspectrometry
AT benaventefernando fastdeterminationofharmalaalkaloidsinediblealgaebycapillaryelectrophoresismassspectrometry
AT sanznebotvictoriam fastdeterminationofharmalaalkaloidsinediblealgaebycapillaryelectrophoresismassspectrometry
AT gagliardileonardogabriel fastdeterminationofharmalaalkaloidsinediblealgaebycapillaryelectrophoresismassspectrometry
bdutipo_str Repositorios
_version_ 1764820454690783232

Ejemplares similares