Immune response to Neospora caninum live tachyzoites in prepubertal female calves
The aim of the present study was to characterize the specific immune response in prepubertal female calves inoculated with Neospora caninum. Forty-eight N. caninum-seronegative 6-month-old Angus female calves were randomly allocated into two groups: group A calves were inoculated subcutaneously (sc)...
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| Autores principales: | , , , , , , , , , , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2019
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/132809 |
| Aporte de: |
| id |
I19-R120-10915-132809 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Veterinaria Ciencias Veterinarias Prepubertal female calves Neospora caninum Live inoculation Immune response |
| spellingShingle |
Veterinaria Ciencias Veterinarias Prepubertal female calves Neospora caninum Live inoculation Immune response Hecker, Y. P. Regidor-Cerrillo, Javier Fiorani, F. Horcajo, Pilar Soria, Ivana Gual, Ignacio Torioni, Susana Campero, Lucía María Echaide, Ignacio Álvarez-García, Gema Ortega-Mora, Luis Miguel Zamorano, Patricia Inés Venturini, María Cecilia Odeón, Anselmo Carlos Cantón, Germán J. Moore, Dadín Prando Immune response to Neospora caninum live tachyzoites in prepubertal female calves |
| topic_facet |
Veterinaria Ciencias Veterinarias Prepubertal female calves Neospora caninum Live inoculation Immune response |
| description |
The aim of the present study was to characterize the specific immune response in prepubertal female calves inoculated with Neospora caninum. Forty-eight N. caninum-seronegative 6-month-old Angus female calves were randomly allocated into two groups: group A calves were inoculated subcutaneously (sc) with 1 × 106 tachyzoites of the low virulence NC-Argentina LP1 isolate in sterile phosphate-buffered saline (PBS); group B calves were mock inoculated sc with sterile PBS. Calves from group A developed a specific immune response characterized by the production of IgG antibodies and the expression of IFN-γ and TNF-α cytokines. Animals did not present any febrile reaction or reactions at the site of inoculation. Although chronic N. caninum infection was developed in 50% of calves of group A after inoculation, according to the presence of antibodies against rNc-SAG4, antigen characteristic of bradyzoites, N. caninum antibodies dropped below the cut-off of ELISA from day 210 post-inoculation onwards. Future trials using the same group of inoculated animals will allow the characterization of the evolution of the immune response during pregnancy and to determine whether the immunization with the local isolate is able to prevent congenital transmission and to protect against heterologous challenges. |
| format |
Articulo Articulo |
| author |
Hecker, Y. P. Regidor-Cerrillo, Javier Fiorani, F. Horcajo, Pilar Soria, Ivana Gual, Ignacio Torioni, Susana Campero, Lucía María Echaide, Ignacio Álvarez-García, Gema Ortega-Mora, Luis Miguel Zamorano, Patricia Inés Venturini, María Cecilia Odeón, Anselmo Carlos Cantón, Germán J. Moore, Dadín Prando |
| author_facet |
Hecker, Y. P. Regidor-Cerrillo, Javier Fiorani, F. Horcajo, Pilar Soria, Ivana Gual, Ignacio Torioni, Susana Campero, Lucía María Echaide, Ignacio Álvarez-García, Gema Ortega-Mora, Luis Miguel Zamorano, Patricia Inés Venturini, María Cecilia Odeón, Anselmo Carlos Cantón, Germán J. Moore, Dadín Prando |
| author_sort |
Hecker, Y. P. |
| title |
Immune response to Neospora caninum live tachyzoites in prepubertal female calves |
| title_short |
Immune response to Neospora caninum live tachyzoites in prepubertal female calves |
| title_full |
Immune response to Neospora caninum live tachyzoites in prepubertal female calves |
| title_fullStr |
Immune response to Neospora caninum live tachyzoites in prepubertal female calves |
| title_full_unstemmed |
Immune response to Neospora caninum live tachyzoites in prepubertal female calves |
| title_sort |
immune response to neospora caninum live tachyzoites in prepubertal female calves |
| publishDate |
2019 |
| url |
http://sedici.unlp.edu.ar/handle/10915/132809 |
| work_keys_str_mv |
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Repositorios |
| _version_ |
1764820454351044608 |