The photosensitizing activity of lumazine using 2'-deoxyguanosine 5'-monophosphate and HeLa cells as targets

Lumazines are an important family of heterocyclic compounds present in biological systems as biosynthetic precursors and/or products of metabolic degradation. Upon UV irradiation, the specific compound called lumazine (pteridine-2,4(1,3H)-dione) is able to generate singlet oxygen (1O2), which is one...

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Detalles Bibliográficos
Autores principales: Denofrio, María Paula, Hatz, Sonja, Lorente, Carolina, Cabrerizo, Franco Martín, Ogilby, Peter R., Thomas, Andrés H.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2009
Materias:
Ciencias Exactas
Física
Química
lumazine
photodynamic effects
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/132781
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-132781
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Física
Química
lumazine
photodynamic effects
spellingShingle Ciencias Exactas
Física
Química
lumazine
photodynamic effects
Denofrio, María Paula
Hatz, Sonja
Lorente, Carolina
Cabrerizo, Franco Martín
Ogilby, Peter R.
Thomas, Andrés H.
The photosensitizing activity of lumazine using 2'-deoxyguanosine 5'-monophosphate and HeLa cells as targets
topic_facet Ciencias Exactas
Física
Química
lumazine
photodynamic effects
description Lumazines are an important family of heterocyclic compounds present in biological systems as biosynthetic precursors and/or products of metabolic degradation. Upon UV irradiation, the specific compound called lumazine (pteridine-2,4(1,3H)-dione) is able to generate singlet oxygen (1O2), which is one of the main chemical species responsible for photodynamic effects. To further assess the photosensitizing capability of lumazine (Lum) experiments were performed using the nucleotide 2′-deoxyguanosine 5′-monophosphate (dGMP) and, independently, cervical cancer cells (HeLa cell line) as targets. In the dGMP experiments, the data revealed that dGMP indeed undergoes oxidation/oxygenation photoinduced by Lum. Moreover, dGMP disappearance proceeds through two competing pathways: (1) electron transfer between dGMP and excited-state Lum (Type I process) and (2) reaction of dGMP with 1O2 produced by Lum (Type II process). The multistep processes involved are convoluted and susceptible to changes in experimental conditions. The independent studies with HeLa cells included fluorescence analysis of cell extracts and phototoxicity experiments performed at the single-cell level. Results showed that, upon Lum uptake and irradiation, photodynamic effects occur. In particular, the mitochondria and cell membrane were perturbed, both of which reflect key stages in cell death. The data reported herein illustrate how the irradiation of an endogenous biological compound can have various effects which, depending on the system, can be manifested in different ways.
format Articulo
Articulo
author Denofrio, María Paula
Hatz, Sonja
Lorente, Carolina
Cabrerizo, Franco Martín
Ogilby, Peter R.
Thomas, Andrés H.
author_facet Denofrio, María Paula
Hatz, Sonja
Lorente, Carolina
Cabrerizo, Franco Martín
Ogilby, Peter R.
Thomas, Andrés H.
author_sort Denofrio, María Paula
title The photosensitizing activity of lumazine using 2'-deoxyguanosine 5'-monophosphate and HeLa cells as targets
title_short The photosensitizing activity of lumazine using 2'-deoxyguanosine 5'-monophosphate and HeLa cells as targets
title_full The photosensitizing activity of lumazine using 2'-deoxyguanosine 5'-monophosphate and HeLa cells as targets
title_fullStr The photosensitizing activity of lumazine using 2'-deoxyguanosine 5'-monophosphate and HeLa cells as targets
title_full_unstemmed The photosensitizing activity of lumazine using 2'-deoxyguanosine 5'-monophosphate and HeLa cells as targets
title_sort photosensitizing activity of lumazine using 2'-deoxyguanosine 5'-monophosphate and hela cells as targets
publishDate 2009
url http://sedici.unlp.edu.ar/handle/10915/132781
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