Role of bacterial pyrroloquinoline quinone in phosphate solubilizing ability and in plant growth promotion on strain <i>Serratia</i> sp. S119

The aim of this study was to analyze if cofactor pyrroquinoline quinone from <i>Serratia</i> sp. S119 is involved in the inorganic phosphate solubilization mechanism and in its ability to promote the plant growth. Site directed mutagenesis was performed to obtain a pqqE- minus mutant of...

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Autores principales: Ludueña, Liliana Mercedes, Anzuay, María Soledad, Angelini, Jorge Guillermo, Barros, Germán, Luna, María Flavia, Monge, María del Pilar, Fabra, Adriana, Taurian, Tania
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Ciencias Exactas
Química
PQQ cofactor
Serratia sp.
Phosphate solubilization
Plant growth promotion
pqqE sequence
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/132692
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-132692
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Química
PQQ cofactor
Serratia sp.
Phosphate solubilization
Plant growth promotion
pqqE sequence
spellingShingle Ciencias Exactas
Química
PQQ cofactor
Serratia sp.
Phosphate solubilization
Plant growth promotion
pqqE sequence
Ludueña, Liliana Mercedes
Anzuay, María Soledad
Angelini, Jorge Guillermo
Barros, Germán
Luna, María Flavia
Monge, María del Pilar
Fabra, Adriana
Taurian, Tania
Role of bacterial pyrroloquinoline quinone in phosphate solubilizing ability and in plant growth promotion on strain <i>Serratia</i> sp. S119
topic_facet Ciencias Exactas
Química
PQQ cofactor
Serratia sp.
Phosphate solubilization
Plant growth promotion
pqqE sequence
description The aim of this study was to analyze if cofactor pyrroquinoline quinone from <i>Serratia</i> sp. S119 is involved in the inorganic phosphate solubilization mechanism and in its ability to promote the plant growth. Site directed mutagenesis was performed to obtain a pqqE- minus mutant of strain <i>Serratia</i> sp. S119. The phosphate solubilization ability, gluconate and PQQ production of the mutant <i>Serratia</i> sp. RSL (pqqE-) was analyzed. Mutant RSL (pqqE-) showed significant decrease in P soluble and gluconic acid levels produced and undetectable levels of PQQ cofactor compared with wild-type strain. Complementation with synthetic PQQ cofactor restored P solubilization and gluconate production reaching the levels produced by wild-type strain. PqqE gene sequence indicated that it is highly conserved within <i>Serratia</i> strains and its product shows conserved motifs found in other PqqE proteins of several bacteria. The effect of the inoculation of the PQQ- mutant on peanut and maize plants was evaluated in pot assays. Plants growth parameters showed no differences among the different treatments indicating that PQQ from <i>Serratia</i> sp. S119 is not involved in the growth promotion of these plants. PQQ cofactor is essential for phosphate solubilization ability of <i>Serratia</i> sp. S119 but is not required for growth promotion of peanut and maize plants.
format Articulo
Articulo
author Ludueña, Liliana Mercedes
Anzuay, María Soledad
Angelini, Jorge Guillermo
Barros, Germán
Luna, María Flavia
Monge, María del Pilar
Fabra, Adriana
Taurian, Tania
author_facet Ludueña, Liliana Mercedes
Anzuay, María Soledad
Angelini, Jorge Guillermo
Barros, Germán
Luna, María Flavia
Monge, María del Pilar
Fabra, Adriana
Taurian, Tania
author_sort Ludueña, Liliana Mercedes
title Role of bacterial pyrroloquinoline quinone in phosphate solubilizing ability and in plant growth promotion on strain <i>Serratia</i> sp. S119
title_short Role of bacterial pyrroloquinoline quinone in phosphate solubilizing ability and in plant growth promotion on strain <i>Serratia</i> sp. S119
title_full Role of bacterial pyrroloquinoline quinone in phosphate solubilizing ability and in plant growth promotion on strain <i>Serratia</i> sp. S119
title_fullStr Role of bacterial pyrroloquinoline quinone in phosphate solubilizing ability and in plant growth promotion on strain <i>Serratia</i> sp. S119
title_full_unstemmed Role of bacterial pyrroloquinoline quinone in phosphate solubilizing ability and in plant growth promotion on strain <i>Serratia</i> sp. S119
title_sort role of bacterial pyrroloquinoline quinone in phosphate solubilizing ability and in plant growth promotion on strain <i>serratia</i> sp. s119
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/132692
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