Antiproliferative and apoptosis-inducing activity of an oxidovanadium(IV) complex with the flavonoid silibinin against osteosarcoma cells

Flavonoids are a large family of polyphenolic compounds synthesized by plants. They display interesting biological effects mainly related to their antioxidant properties. On the other hand, vanadium compounds also exhibit different biological and pharmacological effects in cell culture and in animal...

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Autores principales: León, Ignacio Esteban, Porro, V., Di Virgilio, Ana Laura, Naso, Luciana Gissella, Williams, Patricia Ana María, Bollati-Fogolín, Mariela, Etcheverry, Susana Beatriz
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Ciencias Exactas
Química
Bioquímica
Anticancer drug
MG-63 human osteosarcoma cells
Mechanisms of action
Flavonoids
Vanadium
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/132016
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-132016
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Química
Bioquímica
Anticancer drug
MG-63 human osteosarcoma cells
Mechanisms of action
Flavonoids
Vanadium
spellingShingle Ciencias Exactas
Química
Bioquímica
Anticancer drug
MG-63 human osteosarcoma cells
Mechanisms of action
Flavonoids
Vanadium
León, Ignacio Esteban
Porro, V.
Di Virgilio, Ana Laura
Naso, Luciana Gissella
Williams, Patricia Ana María
Bollati-Fogolín, Mariela
Etcheverry, Susana Beatriz
Antiproliferative and apoptosis-inducing activity of an oxidovanadium(IV) complex with the flavonoid silibinin against osteosarcoma cells
topic_facet Ciencias Exactas
Química
Bioquímica
Anticancer drug
MG-63 human osteosarcoma cells
Mechanisms of action
Flavonoids
Vanadium
description Flavonoids are a large family of polyphenolic compounds synthesized by plants. They display interesting biological effects mainly related to their antioxidant properties. On the other hand, vanadium compounds also exhibit different biological and pharmacological effects in cell culture and in animal models. Since coordination of ligands to metals can improve or change the pharmacological properties, we report herein, for the first time, a detailed study of the mechanisms of action of an oxidovanadium(IV) complex with the flavonoid silibinin, Na₂[VO(silibinin)₂]·6H₂O (VOsil), in a model of the human osteosarcoma derived cell line MG-63. The complex inhibited the viability of osteosarcoma cells in a dose-dependent manner with a greater potency than that of silibinin and oxidovanadium(IV) (p < 0.01), demonstrating the benefit of complexation. Cytotoxicity and genotoxicity studies also showed a concentration effect for VOsil. The increase in the levels of reactive oxygen species and the decrease of the ratio of the amount of reduced glutathione to the amount of oxidized glutathione were involved in the deleterious effects of the complex. Besides, the complex caused cell cycle arrest and activated caspase 3, triggering apoptosis as determined by flow cytometry. As a whole, these results show the main mechanisms of the deleterious effects of VOsil in the osteosarcoma cell line, demonstrating that this complex is a promising compound for cancer treatments.
format Articulo
Articulo
author León, Ignacio Esteban
Porro, V.
Di Virgilio, Ana Laura
Naso, Luciana Gissella
Williams, Patricia Ana María
Bollati-Fogolín, Mariela
Etcheverry, Susana Beatriz
author_facet León, Ignacio Esteban
Porro, V.
Di Virgilio, Ana Laura
Naso, Luciana Gissella
Williams, Patricia Ana María
Bollati-Fogolín, Mariela
Etcheverry, Susana Beatriz
author_sort León, Ignacio Esteban
title Antiproliferative and apoptosis-inducing activity of an oxidovanadium(IV) complex with the flavonoid silibinin against osteosarcoma cells
title_short Antiproliferative and apoptosis-inducing activity of an oxidovanadium(IV) complex with the flavonoid silibinin against osteosarcoma cells
title_full Antiproliferative and apoptosis-inducing activity of an oxidovanadium(IV) complex with the flavonoid silibinin against osteosarcoma cells
title_fullStr Antiproliferative and apoptosis-inducing activity of an oxidovanadium(IV) complex with the flavonoid silibinin against osteosarcoma cells
title_full_unstemmed Antiproliferative and apoptosis-inducing activity of an oxidovanadium(IV) complex with the flavonoid silibinin against osteosarcoma cells
title_sort antiproliferative and apoptosis-inducing activity of an oxidovanadium(iv) complex with the flavonoid silibinin against osteosarcoma cells
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/132016
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