The carbohydrate-binding module of Fragaria × ananassa expansin 2 (CBM-FaExp2) binds to cell wall polysaccharides and decreases cell wall enzyme activities "in vitro"

A putative carbohydrate binding module (CBM) from strawberry (Fragaria × ananassa Duch.) expansin 2 (CBM-FaExp2) was cloned and the encoding protein was over-expressed in Escherichia coli and purified in order to evaluate its capacity to bind different...

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Autores principales:	Nardi, Cristina Fernanda, Escudero, Cristian, Villarreal, Natalia Marina, Martínez, Gustavo Adolfo, Civello, Pedro Marcos
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2013
Materias:	Ciencias Exactas Ciencias Agrarias CBM Cell wall Expansin Hydrolases Strawberry
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/131997
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-131997
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Ciencias Agrarias CBM Cell wall Expansin Hydrolases Strawberry
spellingShingle	Ciencias Exactas Ciencias Agrarias CBM Cell wall Expansin Hydrolases Strawberry Nardi, Cristina Fernanda Escudero, Cristian Villarreal, Natalia Marina Martínez, Gustavo Adolfo Civello, Pedro Marcos The carbohydrate-binding module of <i>Fragaria</i> × <i>ananassa</i> expansin 2 (CBM-FaExp2) binds to cell wall polysaccharides and decreases cell wall enzyme activities "in vitro"
topic_facet	Ciencias Exactas Ciencias Agrarias CBM Cell wall Expansin Hydrolases Strawberry
description	A putative carbohydrate binding module (CBM) from strawberry (<i>Fragaria</i> × ananassa</i> Duch.) expansin 2 (CBM-FaExp2) was cloned and the encoding protein was over-expressed in <i>Escherichia coli</i> and purified in order to evaluate its capacity to bind different cell wall polysaccharides “in vitro”. The protein CBM-FaExp2 bound to microcrystalline cellulose, xylan and pectin with different affinities (K<sub>ad</sub> = 33.6 ± 0.44 mL g⁻¹, K<sub>ad</sub> = 11.37 ± 0.87 mL g⁻¹, K<sub>ad</sub> = 10.4 ± 0.19 mL g⁻¹, respectively). According to “in vitro” enzyme assays, this CBM is able to decrease the activity of cell wall degrading enzymes such as polygalacturonase, endo-glucanase, pectinase and xylanase, probably because the binding of CBM-FaExp2 to the different substrates interferes with enzyme activity. The results suggest that expansins would bind not only cellulose but also a wide range of cell wall polymers.
format	Articulo Articulo
author	Nardi, Cristina Fernanda Escudero, Cristian Villarreal, Natalia Marina Martínez, Gustavo Adolfo Civello, Pedro Marcos
author_facet	Nardi, Cristina Fernanda Escudero, Cristian Villarreal, Natalia Marina Martínez, Gustavo Adolfo Civello, Pedro Marcos
author_sort	Nardi, Cristina Fernanda
title	The carbohydrate-binding module of <i>Fragaria</i> × <i>ananassa</i> expansin 2 (CBM-FaExp2) binds to cell wall polysaccharides and decreases cell wall enzyme activities "in vitro"
title_short	The carbohydrate-binding module of <i>Fragaria</i> × <i>ananassa</i> expansin 2 (CBM-FaExp2) binds to cell wall polysaccharides and decreases cell wall enzyme activities "in vitro"
title_full	The carbohydrate-binding module of <i>Fragaria</i> × <i>ananassa</i> expansin 2 (CBM-FaExp2) binds to cell wall polysaccharides and decreases cell wall enzyme activities "in vitro"
title_fullStr	The carbohydrate-binding module of <i>Fragaria</i> × <i>ananassa</i> expansin 2 (CBM-FaExp2) binds to cell wall polysaccharides and decreases cell wall enzyme activities "in vitro"
title_full_unstemmed	The carbohydrate-binding module of <i>Fragaria</i> × <i>ananassa</i> expansin 2 (CBM-FaExp2) binds to cell wall polysaccharides and decreases cell wall enzyme activities "in vitro"
title_sort	carbohydrate-binding module of <i>fragaria</i> × <i>ananassa</i> expansin 2 (cbm-faexp2) binds to cell wall polysaccharides and decreases cell wall enzyme activities "in vitro"
publishDate	2013
url	http://sedici.unlp.edu.ar/handle/10915/131997
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The carbohydrate-binding module of <i>Fragaria</i> × <i>ananassa</i> expansin 2 (CBM-FaExp2) binds to cell wall polysaccharides and decreases cell wall enzyme activities "in vitro"

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