Technique for the identification of osmophores in flowers of herbarium material (TIOFH)

The histochemical studies that search for osmophores differ in the protocol they follow for fixation and discoloration of the samples, and also in the type and number of stains utilized. Despite these differences, all the studies have one point in common: the use of fresh material either collected d...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Hernández, Marcelo Paulo, Katinas, Liliana
Formato: Articulo
Lenguaje:Inglés
Publicado: 2019
Materias:
Ciencias Agrarias
Biología
Floral osmophores
Protocol
Herbarium material
Histochemical technique
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/131989
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-131989
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Agrarias
Biología
Floral osmophores
Protocol
Herbarium material
Histochemical technique
spellingShingle Ciencias Agrarias
Biología
Floral osmophores
Protocol
Herbarium material
Histochemical technique
Hernández, Marcelo Paulo
Katinas, Liliana
Technique for the identification of osmophores in flowers of herbarium material (TIOFH)
topic_facet Ciencias Agrarias
Biología
Floral osmophores
Protocol
Herbarium material
Histochemical technique
description The histochemical studies that search for osmophores differ in the protocol they follow for fixation and discoloration of the samples, and also in the type and number of stains utilized. Despite these differences, all the studies have one point in common: the use of fresh material either collected directly in the field or cultivated in botanical gardens and greenhouses. This is an obvious limitation for the studies of osmophores. Flower parts of herbarium specimens of different dates of collection and different plant families were exposed to variable times and percentages of discoloration agents and under different stains for finding if it is possible to positively test osmophores in this type of material. We obtained positive results discoloring the samples with ethyl alcohol 96° and sodium hypochlorite, and staining with Lugol, Oil Red O, and Neutral Red (TIOFH). A protocol (TIOFH3) for osmophore testing that combines these three stains into a single procedure is proposed.
format Articulo
Articulo
author Hernández, Marcelo Paulo
Katinas, Liliana
author_facet Hernández, Marcelo Paulo
Katinas, Liliana
author_sort Hernández, Marcelo Paulo
title Technique for the identification of osmophores in flowers of herbarium material (TIOFH)
title_short Technique for the identification of osmophores in flowers of herbarium material (TIOFH)
title_full Technique for the identification of osmophores in flowers of herbarium material (TIOFH)
title_fullStr Technique for the identification of osmophores in flowers of herbarium material (TIOFH)
title_full_unstemmed Technique for the identification of osmophores in flowers of herbarium material (TIOFH)
title_sort technique for the identification of osmophores in flowers of herbarium material (tiofh)
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/131989
work_keys_str_mv AT hernandezmarcelopaulo techniquefortheidentificationofosmophoresinflowersofherbariummaterialtiofh
AT katinasliliana techniquefortheidentificationofosmophoresinflowersofherbariummaterialtiofh
bdutipo_str Repositorios
_version_ 1764820453759647745

Ejemplares similares