Dietary and hormonal effects upon activity of "soluble" protein and particulate fraction of fatty acid desaturation system of rat liver microsomes

Rat liver microsomes were extracted with a buffered 0.15 M KCl and 0.25 M sucrose solution and fractionated by centrifugation into a particulate component and a supernatant containing a protein factor necessary for fatty acid desaturation. The delta 6 fatty acid desaturation activity of the extracte...

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Detalles Bibliográficos
Autores principales: Nervi, Aníbal M., Catalá, Ángel, Brenner, Rodolfo Roberto, Peluffo, Raúl Omar
Formato: Articulo
Lenguaje:Inglés
Publicado: 1975
Materias:
Bioquímica
Ciencias Médicas
Fatty Acid
Diet
Hormonal effects
Desaturation
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/131985
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-131985
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Ciencias Médicas
Fatty Acid
Diet
Hormonal effects
Desaturation
spellingShingle Bioquímica
Ciencias Médicas
Fatty Acid
Diet
Hormonal effects
Desaturation
Nervi, Aníbal M.
Catalá, Ángel
Brenner, Rodolfo Roberto
Peluffo, Raúl Omar
Dietary and hormonal effects upon activity of "soluble" protein and particulate fraction of fatty acid desaturation system of rat liver microsomes
topic_facet Bioquímica
Ciencias Médicas
Fatty Acid
Diet
Hormonal effects
Desaturation
description Rat liver microsomes were extracted with a buffered 0.15 M KCl and 0.25 M sucrose solution and fractionated by centrifugation into a particulate component and a supernatant containing a protein factor necessary for fatty acid desaturation. The delta 6 fatty acid desaturation activity of the extracted microsomes was reduced significantly, and the readdition of the supernatant restored the enzymatic activity to the original value of the whole microsomes. A protein diet or a fat-free diet increased the delta 6 desaturation activity of the whole microsomes. The activating effect was evoked upon the particulate components of the enzymatic desaturation system and not upon the protein factor present in the supernatant. Fasting, refeeding, and refeeding plus glucagon and theophylline treatments of rats also modified the delta 6 desaturation activity of whole liver microsomes. The effect also was evoked on the delta 6 desaturation system tightly bound to the microsomal membrane but not on the protein factor of the supernatant. Accordingly, the protein factor of the supernatant is considered to be different from the cyanide sensitive factor and the desaturase.
format Articulo
Articulo
author Nervi, Aníbal M.
Catalá, Ángel
Brenner, Rodolfo Roberto
Peluffo, Raúl Omar
author_facet Nervi, Aníbal M.
Catalá, Ángel
Brenner, Rodolfo Roberto
Peluffo, Raúl Omar
author_sort Nervi, Aníbal M.
title Dietary and hormonal effects upon activity of "soluble" protein and particulate fraction of fatty acid desaturation system of rat liver microsomes
title_short Dietary and hormonal effects upon activity of "soluble" protein and particulate fraction of fatty acid desaturation system of rat liver microsomes
title_full Dietary and hormonal effects upon activity of "soluble" protein and particulate fraction of fatty acid desaturation system of rat liver microsomes
title_fullStr Dietary and hormonal effects upon activity of "soluble" protein and particulate fraction of fatty acid desaturation system of rat liver microsomes
title_full_unstemmed Dietary and hormonal effects upon activity of "soluble" protein and particulate fraction of fatty acid desaturation system of rat liver microsomes
title_sort dietary and hormonal effects upon activity of "soluble" protein and particulate fraction of fatty acid desaturation system of rat liver microsomes
publishDate 1975
url http://sedici.unlp.edu.ar/handle/10915/131985
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bdutipo_str Repositorios
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