Diversity of aerobic spore-forming bacteria isolated from fresh bee pollen intended for human consumption in Argentina
Bee pollen is the result of the agglutination of pollen grains collected from flowers and mixed with nectar and salivary secretions by honey bees. Bee pollen is a natural product exposed to environmental conditions and also provides a unique microhabitat for yeasts and bacterial communities. We anal...
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| Formato: | Articulo |
| Lenguaje: | Inglés |
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2021
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/131866 |
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I19-R120-10915-131866 |
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dspace |
| institution |
Universidad Nacional de La Plata |
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I-19 |
| repository_str |
R-120 |
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SEDICI (UNLP) |
| language |
Inglés |
| topic |
Biología Ciencias Agrarias Bee pollen Bacillus Brevibacillus Lysinibacillus Paenibacillus Rummeliibacillus Argentina |
| spellingShingle |
Biología Ciencias Agrarias Bee pollen Bacillus Brevibacillus Lysinibacillus Paenibacillus Rummeliibacillus Argentina Alippi, Adriana Mónica Fernández, Leticia A. López, Ana Claudia Diversity of aerobic spore-forming bacteria isolated from fresh bee pollen intended for human consumption in Argentina |
| topic_facet |
Biología Ciencias Agrarias Bee pollen Bacillus Brevibacillus Lysinibacillus Paenibacillus Rummeliibacillus Argentina |
| description |
Bee pollen is the result of the agglutination of pollen grains collected from flowers and mixed with nectar and salivary secretions by honey bees. Bee pollen is a natural product exposed to environmental conditions and also provides a unique microhabitat for yeasts and bacterial communities. We analyzed 30 fresh bee pollen samples obtained from the main producing areas of Argentina to identify aerobic-spore-forming bacteria. We obtained 73 isolates belonging to 16 different species through isolation on selective and differential media, morphological and biochemical tests, and PCR and RFLP analysis of genes encoding 16S rRNA. Our data revealed that Bacillus cereus sensu stricto was the most predominant species (50%), followed by Bacillus megaterium (40%) and Bacillus subtilis (40%), respectively. In a minor proportion, Paenibacillus polymyxa (20%), Paenibacillus larvae (17%), Bacillus pumilus (13%), Bacillus licheniformis (13%), Bacillus amyloliquefaciens (10%), Lysinibacillus sphaericus (7%), Bacillus coagulans (7%), Rummelliibacillus stabekisii (7%), Bacillus thuringiensis (7%), Bacillus clausii (3%), Paenibacillus alvei (3%), Bacillus simplex (3%), and Paenibacillus amylolyticus (3%) were also found. Our results showed that Argentinean bee pollen could transmit honey bee diseases due to the presence of viable spores of P. larvae and also spores of toxicogenic B. cereus s.s. and B. megaterium strains. |
| format |
Articulo Articulo |
| author |
Alippi, Adriana Mónica Fernández, Leticia A. López, Ana Claudia |
| author_facet |
Alippi, Adriana Mónica Fernández, Leticia A. López, Ana Claudia |
| author_sort |
Alippi, Adriana Mónica |
| title |
Diversity of aerobic spore-forming bacteria isolated from fresh bee pollen intended for human consumption in Argentina |
| title_short |
Diversity of aerobic spore-forming bacteria isolated from fresh bee pollen intended for human consumption in Argentina |
| title_full |
Diversity of aerobic spore-forming bacteria isolated from fresh bee pollen intended for human consumption in Argentina |
| title_fullStr |
Diversity of aerobic spore-forming bacteria isolated from fresh bee pollen intended for human consumption in Argentina |
| title_full_unstemmed |
Diversity of aerobic spore-forming bacteria isolated from fresh bee pollen intended for human consumption in Argentina |
| title_sort |
diversity of aerobic spore-forming bacteria isolated from fresh bee pollen intended for human consumption in argentina |
| publishDate |
2021 |
| url |
http://sedici.unlp.edu.ar/handle/10915/131866 |
| work_keys_str_mv |
AT alippiadrianamonica diversityofaerobicsporeformingbacteriaisolatedfromfreshbeepollenintendedforhumanconsumptioninargentina AT fernandezleticiaa diversityofaerobicsporeformingbacteriaisolatedfromfreshbeepollenintendedforhumanconsumptioninargentina AT lopezanaclaudia diversityofaerobicsporeformingbacteriaisolatedfromfreshbeepollenintendedforhumanconsumptioninargentina |
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Repositorios |
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