Study of the Degradation Activity and the Strategies to Promote the Bioavailability of Phenanthrene by Sphingomonas paucimobilis Strain 20006FA

The present study describes the phenanthrene-degrading activity of Sphingomonas paucimobilis 20006FA and its ability to promote the bioavailability of phenanthrene. S. paucimobilis 20006FA was isolated from a phenanthrene-contaminated soil microcosm. The strain...

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Detalles Bibliográficos
Autores principales:	Coppotelli, Bibiana Marina, Ibarrolaza, Agustín, Dias, Romina Laura, Del Panno, María Teresa, Berthe-Corti, Luise, Morelli, Irma Susana
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2010
Materias:	Ciencias Exactas Polycyclic Aromatic Hydrocarbon Phenanthrene Mineral Medium Sphingomonas Sodium Salicylate
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/131864
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-131864
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Polycyclic Aromatic Hydrocarbon Phenanthrene Mineral Medium Sphingomonas Sodium Salicylate
spellingShingle	Ciencias Exactas Polycyclic Aromatic Hydrocarbon Phenanthrene Mineral Medium Sphingomonas Sodium Salicylate Coppotelli, Bibiana Marina Ibarrolaza, Agustín Dias, Romina Laura Del Panno, María Teresa Berthe-Corti, Luise Morelli, Irma Susana Study of the Degradation Activity and the Strategies to Promote the Bioavailability of Phenanthrene by <i>Sphingomonas paucimobilis</i> Strain 20006FA
topic_facet	Ciencias Exactas Polycyclic Aromatic Hydrocarbon Phenanthrene Mineral Medium Sphingomonas Sodium Salicylate
description	The present study describes the phenanthrene-degrading activity of <i>Sphingomonas paucimobilis</i> 20006FA and its ability to promote the bioavailability of phenanthrene. <i>S. paucimobilis</i> 20006FA was isolated from a phenanthrene-contaminated soil microcosm. The strain was able to grow in liquid mineral medium saturated with phenanthrene as the sole carbon source, showing high phenanthrene elimination (52.9% of the supplied phenanthrene within 20 days). The accumulation of 1-hydroxy-2-naphthoic acid and salicylic acid as major phenanthrene metabolites and the capacity of the strain to grow with sodium salicylate as the sole source of carbon and energy indicated that the <i>S. paucimobilis</i> 20006FA possesses a complete phenanthrene degradation pathway. However, under the studied conditions, the strain was able to mineralize only the 10% of the consumed phenanthrene. Investigations on the cell ability to promote bioavailability of phenanthrene showed that the <i>S. paucimobilis</i> strain 20006FA exhibited low cell hydrophobicity (0.13), a pronounced chemotaxis toward phenanthrene, and it was able to reduce the surface tension of mineral liquid medium supplemented with phenanthrene as sole carbon source. Scanning electron micrographs revealed that: (1) in suspension cultures, cells formed flocks and showed small vesicles on the cell surface and (2) cells were also able to adhere to phenanthrene crystals and to produce biofilms. Clearly, the strain seems to exhibit two different mechanisms to enhance phenanthrene bioavailability: biosurfactant production and adhesion to the phenanthrene crystals.
format	Articulo Articulo
author	Coppotelli, Bibiana Marina Ibarrolaza, Agustín Dias, Romina Laura Del Panno, María Teresa Berthe-Corti, Luise Morelli, Irma Susana
author_facet	Coppotelli, Bibiana Marina Ibarrolaza, Agustín Dias, Romina Laura Del Panno, María Teresa Berthe-Corti, Luise Morelli, Irma Susana
author_sort	Coppotelli, Bibiana Marina
title	Study of the Degradation Activity and the Strategies to Promote the Bioavailability of Phenanthrene by <i>Sphingomonas paucimobilis</i> Strain 20006FA
title_short	Study of the Degradation Activity and the Strategies to Promote the Bioavailability of Phenanthrene by <i>Sphingomonas paucimobilis</i> Strain 20006FA
title_full	Study of the Degradation Activity and the Strategies to Promote the Bioavailability of Phenanthrene by <i>Sphingomonas paucimobilis</i> Strain 20006FA
title_fullStr	Study of the Degradation Activity and the Strategies to Promote the Bioavailability of Phenanthrene by <i>Sphingomonas paucimobilis</i> Strain 20006FA
title_full_unstemmed	Study of the Degradation Activity and the Strategies to Promote the Bioavailability of Phenanthrene by <i>Sphingomonas paucimobilis</i> Strain 20006FA
title_sort	study of the degradation activity and the strategies to promote the bioavailability of phenanthrene by <i>sphingomonas paucimobilis</i> strain 20006fa
publishDate	2010
url	http://sedici.unlp.edu.ar/handle/10915/131864
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Study of the Degradation Activity and the Strategies to Promote the Bioavailability of Phenanthrene by <i>Sphingomonas paucimobilis</i> Strain 20006FA

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