Measurement of Ascorbic Acid and Glutathione Content in Cyanobacterium Synechocystis sp. PCC 6803

Ascorbic acid (AsA) and gluthathione (GSH) are two key components of the antioxidant machinery of eukaryotic and prokaryotic cells. The cyanobacterium Synechocystis sp. PCC 6803 presents both compounds in different concentrations (AsA, 20-100 μM and GSH, 2-5 mM). Therefore, it is important to have p...

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Detalles Bibliográficos
Autores principales: Aguillera, Anabella, Steelheart Molina, María Charlotte, Alegre, Matías Leonel, Berdun, Federico, Salerno, Graciela Lidia, Bartoli, Carlos Guillermo, Pagnussat, Gabriela Carolina, Martin, María Victoria
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Ciencias Naturales
Biología
Cyanobacteria
Antioxidants
Glutathione
Ascorbic acid
Oxidative stress
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/131844
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-131844
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Naturales
Biología
Cyanobacteria
Antioxidants
Glutathione
Ascorbic acid
Oxidative stress
spellingShingle Ciencias Naturales
Biología
Cyanobacteria
Antioxidants
Glutathione
Ascorbic acid
Oxidative stress
Aguillera, Anabella
Steelheart Molina, María Charlotte
Alegre, Matías Leonel
Berdun, Federico
Salerno, Graciela Lidia
Bartoli, Carlos Guillermo
Pagnussat, Gabriela Carolina
Martin, María Victoria
Measurement of Ascorbic Acid and Glutathione Content in Cyanobacterium Synechocystis sp. PCC 6803
topic_facet Ciencias Naturales
Biología
Cyanobacteria
Antioxidants
Glutathione
Ascorbic acid
Oxidative stress
description Ascorbic acid (AsA) and gluthathione (GSH) are two key components of the antioxidant machinery of eukaryotic and prokaryotic cells. The cyanobacterium Synechocystis sp. PCC 6803 presents both compounds in different concentrations (AsA, 20-100 μM and GSH, 2-5 mM). Therefore, it is important to have precise and sensitive methods to determine the redox status in the cell and to detect variations in this antioxidants. In this protocol, we describe an improved method to estimate the content of both antioxidants (in their reduced and oxidized forms) from the same sample obtained from liquid cultures of Synechocystis sp. PCC 6803.
format Articulo
Articulo
author Aguillera, Anabella
Steelheart Molina, María Charlotte
Alegre, Matías Leonel
Berdun, Federico
Salerno, Graciela Lidia
Bartoli, Carlos Guillermo
Pagnussat, Gabriela Carolina
Martin, María Victoria
author_facet Aguillera, Anabella
Steelheart Molina, María Charlotte
Alegre, Matías Leonel
Berdun, Federico
Salerno, Graciela Lidia
Bartoli, Carlos Guillermo
Pagnussat, Gabriela Carolina
Martin, María Victoria
author_sort Aguillera, Anabella
title Measurement of Ascorbic Acid and Glutathione Content in Cyanobacterium Synechocystis sp. PCC 6803
title_short Measurement of Ascorbic Acid and Glutathione Content in Cyanobacterium Synechocystis sp. PCC 6803
title_full Measurement of Ascorbic Acid and Glutathione Content in Cyanobacterium Synechocystis sp. PCC 6803
title_fullStr Measurement of Ascorbic Acid and Glutathione Content in Cyanobacterium Synechocystis sp. PCC 6803
title_full_unstemmed Measurement of Ascorbic Acid and Glutathione Content in Cyanobacterium Synechocystis sp. PCC 6803
title_sort measurement of ascorbic acid and glutathione content in cyanobacterium synechocystis sp. pcc 6803
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/131844
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