Feline testicular ultrasonogram differentiates pre vs. postpubertal and normal vs. disrupted spermatogenesis

The aims of this study were: to ultrasonograhically describe and compare testicular parenchyma echogenicity and heterogeneity using digital image analysis in: I) prepubertal (PREP), peripubertal (PERI) and mature (MAT) cats; II) Normal and abnormal mature felids. Secondary, the relationships between...

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Autores principales: D'Francisco, Florencia Alina, Lapuente, Camila, López Merlo, Mariana Lucía, Barbeito, Claudio Gustavo, Gobello, María Cristina
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2020
Materias:
Ciencias Veterinarias
Puberty
Andrology
Subfertility
Ecography
Histology
Germinal epithelium
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/131706
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-131706
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Puberty
Andrology
Subfertility
Ecography
Histology
Germinal epithelium
spellingShingle Ciencias Veterinarias
Puberty
Andrology
Subfertility
Ecography
Histology
Germinal epithelium
D'Francisco, Florencia Alina
Lapuente, Camila
López Merlo, Mariana Lucía
Barbeito, Claudio Gustavo
Gobello, María Cristina
Feline testicular ultrasonogram differentiates pre vs. postpubertal and normal vs. disrupted spermatogenesis
topic_facet Ciencias Veterinarias
Puberty
Andrology
Subfertility
Ecography
Histology
Germinal epithelium
description The aims of this study were: to ultrasonograhically describe and compare testicular parenchyma echogenicity and heterogeneity using digital image analysis in: I) prepubertal (PREP), peripubertal (PERI) and mature (MAT) cats; II) Normal and abnormal mature felids. Secondary, the relationships between histomorphological and ultrasonographic attributes of the testes were also determined. I) Fourteen, PREP, PERI and MAT male cats were ultrasonographically examined and then castrated. II) Seven adult cats were ultrasonographically examined before and after a GnRH antagonist administration and then castrated. All the testes were grossly and histomorphometrically assessed. In the frozen digital images of the longitudinal ultrasound sections, 3 regions of interest (ROI, 1 mm2) were selected. Within each ROI the echogenicity and the heterogeneity of the testicular parenchyma were digitally analyzed. In experiment I, testicular volume (0.15 ± 0.0 vs. 0.49 ± 0.1 vs. 1.65 ± 0.1; P < 0.01) and gonadosomatic index (0.04 ± 0.0 vs. 0.05 ± 0.0 vs. 0.08 ± 0.0; P < 0.01), echogenicity (56.54 ± 0.75 vs. 81.87 ± 5.88 vs.94.67 ± 3.62; P < 0.01) and heterogeneity (10.2420 ± 1.3740 vs.13.65 ± 0.65 vs. 14.67 ± 1.49; P < 0.01) augmented throughout PRE, PERI, and MAT. In experiment II, testicular volume (1.00 ± 0.09 vs. 0.85 ± 0.09; P < 0.05), echogenicity (87.74 ± 1.53 vs. 83.32 ± 1.54; P 0.01) but not heterogeneity (14.09 ± 0.26 vs. 14.19 ± 0.29; P > 0.05) decreased in the post GnRH antagonist abnormal testes. For both experiments, testicular volume, seminiferous tubular diameter, percentage of spermatids as the most mature cell type, and luminal/intertubular ratio were highly correlated (P < 0.01) with their echotextural attributes. Computer-assisted image analysis of B mode ultrasonogram appears as a good indicator of pubertal development and mild alterations of spermatogenesis in felids.
format Articulo
Preprint
author D'Francisco, Florencia Alina
Lapuente, Camila
López Merlo, Mariana Lucía
Barbeito, Claudio Gustavo
Gobello, María Cristina
author_facet D'Francisco, Florencia Alina
Lapuente, Camila
López Merlo, Mariana Lucía
Barbeito, Claudio Gustavo
Gobello, María Cristina
author_sort D'Francisco, Florencia Alina
title Feline testicular ultrasonogram differentiates pre vs. postpubertal and normal vs. disrupted spermatogenesis
title_short Feline testicular ultrasonogram differentiates pre vs. postpubertal and normal vs. disrupted spermatogenesis
title_full Feline testicular ultrasonogram differentiates pre vs. postpubertal and normal vs. disrupted spermatogenesis
title_fullStr Feline testicular ultrasonogram differentiates pre vs. postpubertal and normal vs. disrupted spermatogenesis
title_full_unstemmed Feline testicular ultrasonogram differentiates pre vs. postpubertal and normal vs. disrupted spermatogenesis
title_sort feline testicular ultrasonogram differentiates pre vs. postpubertal and normal vs. disrupted spermatogenesis
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/131706
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