Wheat storage proteins: changes on the glutenins after wheat infection with different isolates of <i>Fusarium graminearum</i>

Wheat gluten proteins are decisive for the industrial properties of flour, so alterations resulting from grain infection with Fusarium graminearum produce changes in the glutenin content that affect the baking properties. This work analyzes the high-molecular-weight glutenin changes from wheat flour...

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Autores principales: Ortega, Leonel Maximiliano, Moure, María Candela, González, Esteban Manuel, Alconada Magliano, Teresa María
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Química
Whole grain flour
Aggressiveness
Fungal infection
Gluten proteins
HPLC analysis
SDS-PAGE analysis
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/131658
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-131658
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Whole grain flour
Aggressiveness
Fungal infection
Gluten proteins
HPLC analysis
SDS-PAGE analysis
spellingShingle Química
Whole grain flour
Aggressiveness
Fungal infection
Gluten proteins
HPLC analysis
SDS-PAGE analysis
Ortega, Leonel Maximiliano
Moure, María Candela
González, Esteban Manuel
Alconada Magliano, Teresa María
Wheat storage proteins: changes on the glutenins after wheat infection with different isolates of <i>Fusarium graminearum</i>
topic_facet Química
Whole grain flour
Aggressiveness
Fungal infection
Gluten proteins
HPLC analysis
SDS-PAGE analysis
description Wheat gluten proteins are decisive for the industrial properties of flour, so alterations resulting from grain infection with Fusarium graminearum produce changes in the glutenin content that affect the baking properties. This work analyzes the high-molecular-weight glutenin changes from wheat flour with different degrees of F. graminearum infection at field, since these proteins are determinant for the quality properties of flour. Wheat cultivars—on field trials—infected with F. graminearum isolates of diverse aggressiveness showed severity values between 9.1 and 42.58% and thousand kernel weight values between 28.12 and 32.33 g. Negative correlations between severity and protein content and positive correlations between yield and protein content were observed, employing reversed-phase high-performance liquid chromatography and polyacrylamide gel electrophoresis. Furthermore, the protein signal changes were in agreement for both methodological approaches. Also, the degree of disease observed and the protein changes on infected wheat cultivars varied in relation with the aggressiveness of the isolate responsible for the infection. The principal component analysis showed a close arrangement among protein values obtained by HPLC. For each cultivar, two principal components were obtained, which explained 80.85%, 88.48%, and 93.33% of the total variance (cultivars Sy200, AGP Fast, and Klein Tigre respectively). To our knowledge, the approaches employed for the analysis of protein changes according to the degree of disease, as well as the thorough statistical analysis, are novel for the study of Fusarium Head Blight.
format Articulo
Articulo
author Ortega, Leonel Maximiliano
Moure, María Candela
González, Esteban Manuel
Alconada Magliano, Teresa María
author_facet Ortega, Leonel Maximiliano
Moure, María Candela
González, Esteban Manuel
Alconada Magliano, Teresa María
author_sort Ortega, Leonel Maximiliano
title Wheat storage proteins: changes on the glutenins after wheat infection with different isolates of <i>Fusarium graminearum</i>
title_short Wheat storage proteins: changes on the glutenins after wheat infection with different isolates of <i>Fusarium graminearum</i>
title_full Wheat storage proteins: changes on the glutenins after wheat infection with different isolates of <i>Fusarium graminearum</i>
title_fullStr Wheat storage proteins: changes on the glutenins after wheat infection with different isolates of <i>Fusarium graminearum</i>
title_full_unstemmed Wheat storage proteins: changes on the glutenins after wheat infection with different isolates of <i>Fusarium graminearum</i>
title_sort wheat storage proteins: changes on the glutenins after wheat infection with different isolates of <i>fusarium graminearum</i>
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/131658
work_keys_str_mv AT ortegaleonelmaximiliano wheatstorageproteinschangesonthegluteninsafterwheatinfectionwithdifferentisolatesofifusariumgraminearumi
AT mouremariacandela wheatstorageproteinschangesonthegluteninsafterwheatinfectionwithdifferentisolatesofifusariumgraminearumi
AT gonzalezestebanmanuel wheatstorageproteinschangesonthegluteninsafterwheatinfectionwithdifferentisolatesofifusariumgraminearumi
AT alconadamaglianoteresamaria wheatstorageproteinschangesonthegluteninsafterwheatinfectionwithdifferentisolatesofifusariumgraminearumi
bdutipo_str Repositorios
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