Co-culture with potentially probiotic microorganisms antagonises virulence factors of Clostridium difficile in vitro

Toxigenic strains of Clostridium difficile were co-cultured with different strains of bifidobacteria and lactobacilli. Spent culture supernatants were tested for biological activity on cultured Vero cells. Co-culture of C. difficile with some potentially probiotic strains lead to a reduction of the...

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Autores principales:	Trejo, Fernando Miguel, Pérez, Pablo Fernando, De Antoni, Graciela Liliana
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2010
Materias:	Ciencias Exactas Bioquímica Bifidobacterium Lactobacillus Clostridium difficile Probiotics Toxins Virulence
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/131490
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-131490
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Bioquímica Bifidobacterium Lactobacillus Clostridium difficile Probiotics Toxins Virulence
spellingShingle	Ciencias Exactas Bioquímica Bifidobacterium Lactobacillus Clostridium difficile Probiotics Toxins Virulence Trejo, Fernando Miguel Pérez, Pablo Fernando De Antoni, Graciela Liliana Co-culture with potentially probiotic microorganisms antagonises virulence factors of Clostridium difficile in vitro
topic_facet	Ciencias Exactas Bioquímica Bifidobacterium Lactobacillus Clostridium difficile Probiotics Toxins Virulence
description	Toxigenic strains of Clostridium difficile were co-cultured with different strains of bifidobacteria and lactobacilli. Spent culture supernatants were tested for biological activity on cultured Vero cells. Co-culture of C. difficile with some potentially probiotic strains lead to a reduction of the biological activity of spent culture supernatants. The observed effects cannot be ascribed either to secreted factors from the probiotic strains or to toxin adsorption by bacterial cells. Immunological assays showed that there was significant diminution of both clostridial toxins (TcdA and TcdB) in spent culture supernatants of co-cultures as compared with pure clostridial cultures. Even though co-cultured clostridial cells showed a slight increase of intracellular toxins, this increase did not completely explains the reduction of toxin concentration in culture supernatants. The evidence suggests that the antagonism could be due to the diminution of the synthesis and/or secretion of both clostridial toxins. Our findings provide new insights into the possible mechanisms involved in the protective effect of probiotics in the context of C. difficile infection.
format	Articulo Articulo
author	Trejo, Fernando Miguel Pérez, Pablo Fernando De Antoni, Graciela Liliana
author_facet	Trejo, Fernando Miguel Pérez, Pablo Fernando De Antoni, Graciela Liliana
author_sort	Trejo, Fernando Miguel
title	Co-culture with potentially probiotic microorganisms antagonises virulence factors of Clostridium difficile in vitro
title_short	Co-culture with potentially probiotic microorganisms antagonises virulence factors of Clostridium difficile in vitro
title_full	Co-culture with potentially probiotic microorganisms antagonises virulence factors of Clostridium difficile in vitro
title_fullStr	Co-culture with potentially probiotic microorganisms antagonises virulence factors of Clostridium difficile in vitro
title_full_unstemmed	Co-culture with potentially probiotic microorganisms antagonises virulence factors of Clostridium difficile in vitro
title_sort	co-culture with potentially probiotic microorganisms antagonises virulence factors of clostridium difficile in vitro
publishDate	2010
url	http://sedici.unlp.edu.ar/handle/10915/131490
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bdutipo_str	Repositorios
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Co-culture with potentially probiotic microorganisms antagonises virulence factors of Clostridium difficile in vitro

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