Determination of heat transfer coefficients in plastic French straws plunged in liquid nitrogen
The knowledge of the thermodynamic process during the cooling of reproductive biological systems is important to assess and optimize the cryopreservation procedures. The time–temperature curve of a sample immersed in liquid nitrogen enables the calculation of cooling rates and helps to determine whe...
Guardado en:
| Autores principales: | , , , |
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| Formato: | Articulo Preprint |
| Lenguaje: | Inglés |
| Publicado: |
2014
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/131333 |
| Aporte de: |
| id |
I19-R120-10915-131333 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Exactas Química Surface heat transfer coefficient Liquid nitrogen Freezing Cooling French straws Nucleate pool boiling Finite element method Film pool boiling Boiling correlation |
| spellingShingle |
Ciencias Exactas Química Surface heat transfer coefficient Liquid nitrogen Freezing Cooling French straws Nucleate pool boiling Finite element method Film pool boiling Boiling correlation Santos, María Victoria Sansiñena, Marina Chirife, Jorge Zaritzky, Noemí Elisabet Determination of heat transfer coefficients in plastic French straws plunged in liquid nitrogen |
| topic_facet |
Ciencias Exactas Química Surface heat transfer coefficient Liquid nitrogen Freezing Cooling French straws Nucleate pool boiling Finite element method Film pool boiling Boiling correlation |
| description |
The knowledge of the thermodynamic process during the cooling of reproductive biological systems is important to assess and optimize the cryopreservation procedures. The time–temperature curve of a sample immersed in liquid nitrogen enables the calculation of cooling rates and helps to determine whether it is vitrified or undergoes phase change transition. When dealing with cryogenic liquids, the temperature difference between the solid and the sample is high enough to cause boiling of the liquid, and the sample can undergo different regimes such as film and/or nucleate pool boiling.
In the present work, the surface heat transfer coefficients (h) for plastic French straws plunged in liquid nitrogen were determined using the measurement of time–temperature curves. When straws filled with ice were used the cooling curve showed an abrupt slope change which was attributed to the transition of film into nucleate pool boiling regime. The h value that fitted each stage of the cooling process was calculated using a numerical finite element program that solves the heat transfer partial differential equation under transient conditions. In the cooling process corresponding to film boiling regime, the h that best fitted experimental results was h = 148.12 ± 5.4 W/m2 K and for nucleate-boiling h = 1355 ± 51 W/m2 K. These values were further validated by predicting the time–temperature curve for French straws filled with a biological fluid system (bovine semen-extender) which undergoes freezing. Good agreement was obtained between the experimental and predicted temperature profiles, further confirming the accuracy of the h values previously determined for the ice-filled straw. These coefficients were corroborated using literature correlations.
The determination of the boiling regimes that govern the cooling process when plunging straws in liquid nitrogen constitutes an important issue when trying to optimize cryopreservation procedures. Furthermore, this information can lead to improvements in the design of cooling devices in the cryobiology field. |
| format |
Articulo Preprint |
| author |
Santos, María Victoria Sansiñena, Marina Chirife, Jorge Zaritzky, Noemí Elisabet |
| author_facet |
Santos, María Victoria Sansiñena, Marina Chirife, Jorge Zaritzky, Noemí Elisabet |
| author_sort |
Santos, María Victoria |
| title |
Determination of heat transfer coefficients in plastic French straws plunged in liquid nitrogen |
| title_short |
Determination of heat transfer coefficients in plastic French straws plunged in liquid nitrogen |
| title_full |
Determination of heat transfer coefficients in plastic French straws plunged in liquid nitrogen |
| title_fullStr |
Determination of heat transfer coefficients in plastic French straws plunged in liquid nitrogen |
| title_full_unstemmed |
Determination of heat transfer coefficients in plastic French straws plunged in liquid nitrogen |
| title_sort |
determination of heat transfer coefficients in plastic french straws plunged in liquid nitrogen |
| publishDate |
2014 |
| url |
http://sedici.unlp.edu.ar/handle/10915/131333 |
| work_keys_str_mv |
AT santosmariavictoria determinationofheattransfercoefficientsinplasticfrenchstrawsplungedinliquidnitrogen AT sansinenamarina determinationofheattransfercoefficientsinplasticfrenchstrawsplungedinliquidnitrogen AT chirifejorge determinationofheattransfercoefficientsinplasticfrenchstrawsplungedinliquidnitrogen AT zaritzkynoemielisabet determinationofheattransfercoefficientsinplasticfrenchstrawsplungedinliquidnitrogen |
| bdutipo_str |
Repositorios |
| _version_ |
1764820453670518784 |