Nutraceutical emulsion containing valproic acid (NE-VPA): a drug delivery system for reversion of seizures in zebrafish larvae epilepsy model

Valproic acid (VPA) is an antiepileptic drug, which is currently used in neurodegenerative diseases. However, a high dose is required to obtain a therapeutic effect. Long-chain polyunsaturated fatty acids (PUFAs), such as omega 3 and omega 6, are efficient complements in treatments for neurological...

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Autores principales: Feas, Daniela Agustina, Igartúa, Daniela Edith, Calienni, María Natalia, Martinez, Carolina Soledad, Pifano, Marina, Chiaramoni, Nadia Silvia, Alonso, Silvia del Valle, Prieto, María Jimena
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Biología
Epilepsy
Valproic acid
Emulsion
Oral drug delivery
Toxicity
Zebrafish
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/131271
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-131271
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Epilepsy
Valproic acid
Emulsion
Oral drug delivery
Toxicity
Zebrafish
spellingShingle Biología
Epilepsy
Valproic acid
Emulsion
Oral drug delivery
Toxicity
Zebrafish
Feas, Daniela Agustina
Igartúa, Daniela Edith
Calienni, María Natalia
Martinez, Carolina Soledad
Pifano, Marina
Chiaramoni, Nadia Silvia
Alonso, Silvia del Valle
Prieto, María Jimena
Nutraceutical emulsion containing valproic acid (NE-VPA): a drug delivery system for reversion of seizures in zebrafish larvae epilepsy model
topic_facet Biología
Epilepsy
Valproic acid
Emulsion
Oral drug delivery
Toxicity
Zebrafish
description Valproic acid (VPA) is an antiepileptic drug, which is currently used in neurodegenerative diseases. However, a high dose is required to obtain a therapeutic effect. Long-chain polyunsaturated fatty acids (PUFAs), such as omega 3 and omega 6, are efficient complements in treatments for neurological diseases. Previous studies have reported that a dietary supplement containing PUFAs together with the administration of antiepileptic drugs significantly reduces the frequency of seizures. Based on this, the main goal of this work was to obtain a complex based on VPA encapsulation in an oil/water (o/w) nutraceutical emulsion (NE) enriched with PUFAs for oral administration. Besides, encapsulation of VPA might reduce its dose and increase its therapeutic effect. In order to study its effect, we used a zebrafish larvae model of induced epileptiform behavior with the proconvulsant drug pentylenetetrazol (PTZ). Results have shown that when 100 μM VPA and fatty acids were combined in the NE (NE-VPA), the epileptiform behavior of PTZ-treated zebrafish larvae decreased significantly. Additionally, morphological changes, hepatotoxicity, lethality and heart rate were studied. Despite the fact that a high dose of VPA exerted a cardiotoxic effect, this was no longer detected after addition of this drug in the NE. This treatment exerted a significant antiepileptic effect and did not result in highly toxic or lethal effects. In order to develop an improved pharmaceutical treatment, and considering that all the components used are FDA approved for consumption, the NE-VPA selected might be easily incorporated into clinical trials.
format Articulo
Articulo
author Feas, Daniela Agustina
Igartúa, Daniela Edith
Calienni, María Natalia
Martinez, Carolina Soledad
Pifano, Marina
Chiaramoni, Nadia Silvia
Alonso, Silvia del Valle
Prieto, María Jimena
author_facet Feas, Daniela Agustina
Igartúa, Daniela Edith
Calienni, María Natalia
Martinez, Carolina Soledad
Pifano, Marina
Chiaramoni, Nadia Silvia
Alonso, Silvia del Valle
Prieto, María Jimena
author_sort Feas, Daniela Agustina
title Nutraceutical emulsion containing valproic acid (NE-VPA): a drug delivery system for reversion of seizures in zebrafish larvae epilepsy model
title_short Nutraceutical emulsion containing valproic acid (NE-VPA): a drug delivery system for reversion of seizures in zebrafish larvae epilepsy model
title_full Nutraceutical emulsion containing valproic acid (NE-VPA): a drug delivery system for reversion of seizures in zebrafish larvae epilepsy model
title_fullStr Nutraceutical emulsion containing valproic acid (NE-VPA): a drug delivery system for reversion of seizures in zebrafish larvae epilepsy model
title_full_unstemmed Nutraceutical emulsion containing valproic acid (NE-VPA): a drug delivery system for reversion of seizures in zebrafish larvae epilepsy model
title_sort nutraceutical emulsion containing valproic acid (ne-vpa): a drug delivery system for reversion of seizures in zebrafish larvae epilepsy model
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/131271
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bdutipo_str Repositorios
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