A method for the purification of bacterial flagellin that allows simple upscaling

There is a growing interest in enterobacterial flagellins that may result in a demand to produce flagellin on an industrial scale for possible applications as an adjuvant, immunomodulatory agent or vaccine antigen. Traditionally, small-scale production of flagellin has occurred in the laboratory by...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hiriart, Yanina, Errea, Agustina Juliana, González Maciel, María Dolores, Lozano López, Juan Carlos, Rumbo, Martín
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2012
Materias:	Ciencias Exactas Flagellin Scaling up Cross-flow filtration Innate response
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/131216
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-131216
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Flagellin Scaling up Cross-flow filtration Innate response
spellingShingle	Ciencias Exactas Flagellin Scaling up Cross-flow filtration Innate response Hiriart, Yanina Errea, Agustina Juliana González Maciel, María Dolores Lozano López, Juan Carlos Rumbo, Martín A method for the purification of bacterial flagellin that allows simple upscaling
topic_facet	Ciencias Exactas Flagellin Scaling up Cross-flow filtration Innate response
description	There is a growing interest in enterobacterial flagellins that may result in a demand to produce flagellin on an industrial scale for possible applications as an adjuvant, immunomodulatory agent or vaccine antigen. Traditionally, small-scale production of flagellin has occurred in the laboratory by flagellar shearing of bacterial surfaces and subsequent ultracentrifugation. The main drawback of this method is the need to use low-agitation cultures to avoid the loss of flagella due to shearing during culture. In the present work, we describe a scalable protocol for the production of flagellin with higher yields than traditional laboratory-scale protocols. The use of cross-flow filtration to concentrate bacterial cultures combines extensive shearing of flagella with a reduction in volume, greatly simplifying downstream processing. This technique also allows the use of highly-agitated culture conditions because any sheared flagella are retained in the bacterial concentrate. Flagella obtained with this procedure showed in vivo and in vitro innate activating capacities similar to those of flagella produced at laboratory scale. This procedure is flexible, allowing an increase in production scale, an enhancement of flagellin yield and no requirement for expensive equipment.
format	Articulo Articulo
author	Hiriart, Yanina Errea, Agustina Juliana González Maciel, María Dolores Lozano López, Juan Carlos Rumbo, Martín
author_facet	Hiriart, Yanina Errea, Agustina Juliana González Maciel, María Dolores Lozano López, Juan Carlos Rumbo, Martín
author_sort	Hiriart, Yanina
title	A method for the purification of bacterial flagellin that allows simple upscaling
title_short	A method for the purification of bacterial flagellin that allows simple upscaling
title_full	A method for the purification of bacterial flagellin that allows simple upscaling
title_fullStr	A method for the purification of bacterial flagellin that allows simple upscaling
title_full_unstemmed	A method for the purification of bacterial flagellin that allows simple upscaling
title_sort	method for the purification of bacterial flagellin that allows simple upscaling
publishDate	2012
url	http://sedici.unlp.edu.ar/handle/10915/131216
work_keys_str_mv	AT hiriartyanina amethodforthepurificationofbacterialflagellinthatallowssimpleupscaling AT erreaagustinajuliana amethodforthepurificationofbacterialflagellinthatallowssimpleupscaling AT gonzalezmacielmariadolores amethodforthepurificationofbacterialflagellinthatallowssimpleupscaling AT lozanolopezjuancarlos amethodforthepurificationofbacterialflagellinthatallowssimpleupscaling AT rumbomartin amethodforthepurificationofbacterialflagellinthatallowssimpleupscaling AT hiriartyanina methodforthepurificationofbacterialflagellinthatallowssimpleupscaling AT erreaagustinajuliana methodforthepurificationofbacterialflagellinthatallowssimpleupscaling AT gonzalezmacielmariadolores methodforthepurificationofbacterialflagellinthatallowssimpleupscaling AT lozanolopezjuancarlos methodforthepurificationofbacterialflagellinthatallowssimpleupscaling AT rumbomartin methodforthepurificationofbacterialflagellinthatallowssimpleupscaling
bdutipo_str	Repositorios
_version_	1764820453469192192

A method for the purification of bacterial flagellin that allows simple upscaling

Ejemplares similares