Influence of vintage, geographic location and cultivar on the structure of microbial communities associated with the grapevine rhizosphere in vineyards of San Juan Province, Argentina

Soil microbiomes, as a primary reservoir for plant colonizing fungi and bacteria, play a major role in determining plant productivity and preventing invasion by pathogenic microorganisms. The use of 16S rRNA and ITS high-throughput amplicon sequencing for analysis of complex microbial communities ha...

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Autores principales: Oyuela Aguilar, Mónica María, Gobbi, Alex, Browne, Patrick D., Ellegaard-Jensen, Lea, Hansen, Lars Hestbjerg, Semorile, Liliana Carmen, Pistorio, Mariano
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Biología
Influence of vintage
Microbial communities
Vineyard location
Cultivar and soil characteristics
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/131184
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-131184
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Influence of vintage
Microbial communities
Vineyard location
Cultivar and soil characteristics
spellingShingle Biología
Influence of vintage
Microbial communities
Vineyard location
Cultivar and soil characteristics
Oyuela Aguilar, Mónica María
Gobbi, Alex
Browne, Patrick D.
Ellegaard-Jensen, Lea
Hansen, Lars Hestbjerg
Semorile, Liliana Carmen
Pistorio, Mariano
Influence of vintage, geographic location and cultivar on the structure of microbial communities associated with the grapevine rhizosphere in vineyards of San Juan Province, Argentina
topic_facet Biología
Influence of vintage
Microbial communities
Vineyard location
Cultivar and soil characteristics
description Soil microbiomes, as a primary reservoir for plant colonizing fungi and bacteria, play a major role in determining plant productivity and preventing invasion by pathogenic microorganisms. The use of 16S rRNA and ITS high-throughput amplicon sequencing for analysis of complex microbial communities have increased dramatically in recent years, establishing links between wine specificity and, environmental and viticultural factors, which are framed into the elusive terroir concept. Given the diverse and complex role these factors play on microbial soil structuring of agricultural crops, the main aim of this study is to evaluate how external factors, such as vintage, vineyard location, cultivar and soil characteristics, may affect the diversity of the microbial communities present. Additionally, we aim to compare the influence these factors have on the structuring of bacterial and fungal populations associated with Malbec grapevine rhizosphere with that of the more widespread Cabernet Sauvignon grapevine cultivar. Samples were taken from Malbec and Cabernet Sauvignon cultivars from two different vineyards in the San Juan Province of Argentina. Total DNA extracts from the rhizosphere soil samples were sequenced using Illumina’s Miseq technology, targeting the V3-V4 hypervariable 16S rRNA region in prokaryotes and the ITS1 region in yeasts. The major bacterial taxa identified were Proteobacteria, Bacteroidetes and Firmicutes, while the major fungal taxa were Ascomycetes, Basidiomycetes, Mortierellomycetes and a low percentage of Glomeromycetes. Significant differences in microbial community composition were found between vintages and vineyard locations, whose soils showed variances in pH, organic matter, and content of carbon, nitrogen, and absorbable phosphorus.
format Articulo
Articulo
author Oyuela Aguilar, Mónica María
Gobbi, Alex
Browne, Patrick D.
Ellegaard-Jensen, Lea
Hansen, Lars Hestbjerg
Semorile, Liliana Carmen
Pistorio, Mariano
author_facet Oyuela Aguilar, Mónica María
Gobbi, Alex
Browne, Patrick D.
Ellegaard-Jensen, Lea
Hansen, Lars Hestbjerg
Semorile, Liliana Carmen
Pistorio, Mariano
author_sort Oyuela Aguilar, Mónica María
title Influence of vintage, geographic location and cultivar on the structure of microbial communities associated with the grapevine rhizosphere in vineyards of San Juan Province, Argentina
title_short Influence of vintage, geographic location and cultivar on the structure of microbial communities associated with the grapevine rhizosphere in vineyards of San Juan Province, Argentina
title_full Influence of vintage, geographic location and cultivar on the structure of microbial communities associated with the grapevine rhizosphere in vineyards of San Juan Province, Argentina
title_fullStr Influence of vintage, geographic location and cultivar on the structure of microbial communities associated with the grapevine rhizosphere in vineyards of San Juan Province, Argentina
title_full_unstemmed Influence of vintage, geographic location and cultivar on the structure of microbial communities associated with the grapevine rhizosphere in vineyards of San Juan Province, Argentina
title_sort influence of vintage, geographic location and cultivar on the structure of microbial communities associated with the grapevine rhizosphere in vineyards of san juan province, argentina
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/131184
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